Enzymes of Polyamine Metabolism, Volume 715 highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Convergence of MYC and the hypusine circuits in the control of metabolism, HDAC10 structure and enzymology, TR-FRET Assay for Profiling HDAC10 Inhibitors and PROTACs, Polyamine Transport Inhibitors: Methods and caveats associated with measuring polyamine uptake in mammalian cells, Evaluation of platinum drug toxicity resulting from polyamine catabolism, Measurements of acrolein adducts resulting from polyamine catabolism, Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori: implications for gastric inflammation and carcinogenesis, and much more.
Other sections delve into Molecular and biochemical analysis of eIF5A, Analysis of in vivo SMOX activity, Structure and role of ATP13A2 in polyamine transport, Analysis of Translational Regulation Using Polysome Profiling and Puromycin Incorporation, Targeting polyamine metabolism in an ex vivo prostatectomy model, Development and characterization of a Drosophila model of SRS, Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples, Gene replacement therapy to restore polyamine metabolism, Investigation of polyamine metabolism in bone homeostasis, Collection, preparation, and biobanking of clinical specimens for polyamine analysis, and many other topics.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xxiii 7. Preface - p. xxix 8. Chapter One: Genetic analyses of Myc and hypusine circuits in tumorigenesis - p. 1 9. Chapter Two: Expression, purification, and crystallization of “humanized” Danio rerio histone deacetylase 10 “HDAC10”, the eukaryotic polyamine deacetylase - p. 19 10. Chapter Three: TR-FRET assay for profiling HDAC10 inhibitors and PROTACs - p. 41 11. Chapter Four: Polyamine transport inhibitors: Methods and caveats associated with measuring polyamine uptake in mammalian cells - p. 65 12. Chapter Five: Evaluation of platinum drug toxicity resulting from polyamine catabolism - p. 93 13. Chapter Six: Measurements of acrolein adducts resulting from polyamine catabolism - p. 117 14. Chapter Seven: Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori: Implications for gastric inflammation and carcinogenesis - p. 137 15. Chapter Eight: Yeast reconstituted translation assays for analysis of eIF5A function - p. 155 16. Chapter Nine: Quantification of spermine oxidase (SMOX) activity in tissues by HPLC - p. 183 17. Chapter Ten: Direct measurement of ATP13A2 polyamine-dependent ATPase activity following rapid purification of lysosomes - p. 201 18. Chapter Eleven: Analysis of translational regulation using polysome profiling and puromycin incorporation - p. 211 19. Chapter Twelve: Targeting polyamine metabolism in an ex vivo prostatectomy model - p. 231 20. Chapter Thirteen: Development and characterization of a Drosophila model of Snyder-Robinson syndrome - p. 241 21. Chapter Fourteen: Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples - p. 257 22. Chapter Fifteen: Gene replacement therapy to restore polyamine metabolism in a Snyder-Robinson syndrome mouse model - p. 271 23. Chapter Sixteen: Methods to study polyamine metabolism during osteogenesis - p. 293 24. Chapter Seventeen: Collection, preparation, and biobanking of clinical specimens for analysis in polyaminopathies - p. 309 25. Chapter Eighteen: An enhanced method for the determination of polyamine content in biological samples by dansyl chloride derivatization and HPLC - p. 323 26. Chapter Nineteen: Cell-based determination of HDAC10-mediated polyamine deacetylase activity - p. 337 27. Chapter Twenty: Drosophila melanogaster imaginal disc assays to study the polyamine transport system - p. 351 28. Chapter Twenty One: A fluorescence-based assay for measuring aminopropyltransferase activity - p. 363 29. Chapter Twenty Two: Isolation of Hafnia paralvei FB315, a polyamine-high-producing bacterium, from aged cheese - p. 389 30. Chapter Twenty Three: Methylated polyamines derivatives and antizyme-related effects - p. 407 31. Chapter Twenty Four: Novel LC-MS/MS assay to quantify D,L-alpha-difluoromethylornithine (DFMO) in mouse plasma - p. 423 32. Chapter Twenty Five: Polyamine quantitation by LC-MS using isobutyl chloroformate derivatives - p. 437
Enzymes of Polyamine Metabolism, Volume 715 highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Convergence of MYC and the hypusine circuits in the control of metabolism, HDAC10 structure and enzymology, TR-FRET Assay for Profiling HDAC10 Inhibitors and PROTACs, Polyamine Transport Inhibitors: Methods and caveats associated with measuring polyamine uptake in mammalian cells, Evaluation of platinum drug toxicity resulting from polyamine catabolism, Measurements of acrolein adducts resulting from polyamine catabolism, Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori: implications for gastric inflammation and carcinogenesis, and much more.
Other sections delve into Molecular and biochemical analysis of eIF5A, Analysis of in vivo SMOX activity, Structure and role of ATP13A2 in polyamine transport, Analysis of Translational Regulation Using Polysome Profiling and Puromycin Incorporation, Targeting polyamine metabolism in an ex vivo prostatectomy model, Development and characterization of a Drosophila model of SRS, Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples, Gene replacement therapy to restore polyamine metabolism, Investigation of polyamine metabolism in bone homeostasis, Collection, preparation, and biobanking of clinical specimens for polyamine analysis, and many other topics.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xxiii 7. Preface - p. xxix 8. Chapter One: Genetic analyses of Myc and hypusine circuits in tumorigenesis - p. 1 9. Chapter Two: Expression, purification, and crystallization of “humanized” Danio rerio histone deacetylase 10 “HDAC10”, the eukaryotic polyamine deacetylase - p. 19 10. Chapter Three: TR-FRET assay for profiling HDAC10 inhibitors and PROTACs - p. 41 11. Chapter Four: Polyamine transport inhibitors: Methods and caveats associated with measuring polyamine uptake in mammalian cells - p. 65 12. Chapter Five: Evaluation of platinum drug toxicity resulting from polyamine catabolism - p. 93 13. Chapter Six: Measurements of acrolein adducts resulting from polyamine catabolism - p. 117 14. Chapter Seven: Alterations in polyamine metabolism induced by the pathogen Helicobacter pylori: Implications for gastric inflammation and carcinogenesis - p. 137 15. Chapter Eight: Yeast reconstituted translation assays for analysis of eIF5A function - p. 155 16. Chapter Nine: Quantification of spermine oxidase (SMOX) activity in tissues by HPLC - p. 183 17. Chapter Ten: Direct measurement of ATP13A2 polyamine-dependent ATPase activity following rapid purification of lysosomes - p. 201 18. Chapter Eleven: Analysis of translational regulation using polysome profiling and puromycin incorporation - p. 211 19. Chapter Twelve: Targeting polyamine metabolism in an ex vivo prostatectomy model - p. 231 20. Chapter Thirteen: Development and characterization of a Drosophila model of Snyder-Robinson syndrome - p. 241 21. Chapter Fourteen: Monitoring ODC activity and polyamines in Bachmann-Bupp syndrome patient biological samples - p. 257 22. Chapter Fifteen: Gene replacement therapy to restore polyamine metabolism in a Snyder-Robinson syndrome mouse model - p. 271 23. Chapter Sixteen: Methods to study polyamine metabolism during osteogenesis - p. 293 24. Chapter Seventeen: Collection, preparation, and biobanking of clinical specimens for analysis in polyaminopathies - p. 309 25. Chapter Eighteen: An enhanced method for the determination of polyamine content in biological samples by dansyl chloride derivatization and HPLC - p. 323 26. Chapter Nineteen: Cell-based determination of HDAC10-mediated polyamine deacetylase activity - p. 337 27. Chapter Twenty: Drosophila melanogaster imaginal disc assays to study the polyamine transport system - p. 351 28. Chapter Twenty One: A fluorescence-based assay for measuring aminopropyltransferase activity - p. 363 29. Chapter Twenty Two: Isolation of Hafnia paralvei FB315, a polyamine-high-producing bacterium, from aged cheese - p. 389 30. Chapter Twenty Three: Methylated polyamines derivatives and antizyme-related effects - p. 407 31. Chapter Twenty Four: Novel LC-MS/MS assay to quantify D,L-alpha-difluoromethylornithine (DFMO) in mouse plasma - p. 423 32. Chapter Twenty Five: Polyamine quantitation by LC-MS using isobutyl chloroformate derivatives - p. 437
