Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter 1: Protein-retention expansion microscopy: Improved sub-cellular imaging resolution through physical specimen expansion - p. page_1 8. Chapter 2: Expansion STED microscopy (ExSTED) - p. page_15 9. Chapter 3: Simple multi-color super-resolution by X10 microscopy - p. page_33 10. Chapter 4: Ultrastructure expansion microscopy (U-ExM) - p. page_57 11. Chapter 5: Expansion microscopy imaging of various neuronal structures - p. page_83 12. Chapter 6: Mapping the neuronal cytoskeleton using expansion microscopy - p. page_105 13. Chapter 7: Mechanical expansion microscopy - p. page_125 14. Chapter 8: Enhanced expansion microscopy to measure nanoscale structural and biochemical remodeling in single cells - p. page_147 15. Chapter 9: Application of expansion microscopy on developing Arabidopsis seeds - p. page_181 16. Chapter 10: A protocol to expand plant nuclei - p. page_197 17. Chapter 11: Expansion microscopy on Drosophila spermatocyte centrioles - p. page_217 18. Chapter 12: Expansion microscopy of the mitotic spindle - p. page_247 19. Chapter 13: Expansion microscopy at the nanoscale: The nuclear pore complex as a fiducial landmark - p. page_275 20. Chapter 14: Improving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy - p. page_297 21. Chapter 15: Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures - p. page_317
Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter 1: Protein-retention expansion microscopy: Improved sub-cellular imaging resolution through physical specimen expansion - p. page_1 8. Chapter 2: Expansion STED microscopy (ExSTED) - p. page_15 9. Chapter 3: Simple multi-color super-resolution by X10 microscopy - p. page_33 10. Chapter 4: Ultrastructure expansion microscopy (U-ExM) - p. page_57 11. Chapter 5: Expansion microscopy imaging of various neuronal structures - p. page_83 12. Chapter 6: Mapping the neuronal cytoskeleton using expansion microscopy - p. page_105 13. Chapter 7: Mechanical expansion microscopy - p. page_125 14. Chapter 8: Enhanced expansion microscopy to measure nanoscale structural and biochemical remodeling in single cells - p. page_147 15. Chapter 9: Application of expansion microscopy on developing Arabidopsis seeds - p. page_181 16. Chapter 10: A protocol to expand plant nuclei - p. page_197 17. Chapter 11: Expansion microscopy on Drosophila spermatocyte centrioles - p. page_217 18. Chapter 12: Expansion microscopy of the mitotic spindle - p. page_247 19. Chapter 13: Expansion microscopy at the nanoscale: The nuclear pore complex as a fiducial landmark - p. page_275 20. Chapter 14: Improving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy - p. page_297 21. Chapter 15: Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures - p. page_317
