Fluorine Metabolism, Transport and Enzymatic Chemistry, Volume 696 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting topics on Fluorine Metabolism, Transport, and Enzymatic Chemistry. Chapters in this new release include Determination of fluoride sensitivity and resistance in oral biofilm models, NMR methods to detect fluoride binding and transport by membrane proteins, Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans, Measurement of fluoride efflux from lipid vesicles and cells, Defluorination of PFAS by Acidimicrobium sp. A6 and Potential Applications for Remediation, and more.
Additional chapters cover Computational approaches to investigate F- binding, selectivity and transport in F- exporters, Defluorination as a key trait to gauge the biodegradability of fluorinated pollutants: challenges and opportunities, Patch-Clamp Electrophysiology of Fluoride Channels in Saccharomyces cerevisiae, and Synthesis of fluorophenylalanine-encoded proteins in HEK cells.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 - p. p21 9. Fluoride detection, transport and resistance - p. p21 10. Chapter One: Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans - p. 4 11. Chapter Two: NMR methods to detect fluoride binding and transport by membrane proteins - p. 26 12. Chapter Three: Lanthanum-fluoride electrode-based methods to monitor fluoride transport in cells and reconstituted lipid vesicles - p. 44 13. Chapter Four: Microwell fluoride assay screening for enzymatic defluorination - p. 66 14. Chapter Five: Direct fluoride monitoring using a fluorogenic RNA-based biosensor - p. 86 15. Chapter Six: Computational approaches to investigate fluoride binding, selectivity and transport across the membrane - p. 110 16. Chapter Seven: Measurement and analysis of microbial fluoride resistance in dental biofilm models - p. 157 17. Section 2 - p. 172 18. Section 2 Enzymatic fluorination - p. 172 19. Chapter Eight: Preparation, assay, and application of 4-fluorothreonine transaldolase from Streptomyces sp. MA37 for β-hydroxyl amino acid derivatives - p. 178 20. Chapter Nine: Synthesis of fluorinated amino acids by low-specificity, promiscuous aldolases coupled to in situ fluorodonor generation - p. 201 21. Chapter Ten: Radical fluorine transfer catalysed by an engineered nonheme iron enzyme - p. 232 22. Section 3 - p. 246 23. Section 3 Biodegradation of organofluorine compounds - p. 246 24. Chapter Eleven: Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans - p. 253 25. Chapter Twelve: Defluorination of PFAS by Acidimicrobium sp. strain A6 and potential applications for remediation - p. 288 26. Chapter Thirteen: Defluorination as the key trait to gauge the biodegradability of fluorinated pollutants in environmental microbial communities - p. 322 27. Section 4 - p. 336 28. Section 4 Fluorine as a biochemical probe - p. 336 29. Chapter Fourteen: Expression and purification of fluorinated proteins from mammalian suspension culture - p. 342
Fluorine Metabolism, Transport and Enzymatic Chemistry, Volume 696 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting topics on Fluorine Metabolism, Transport, and Enzymatic Chemistry. Chapters in this new release include Determination of fluoride sensitivity and resistance in oral biofilm models, NMR methods to detect fluoride binding and transport by membrane proteins, Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans, Measurement of fluoride efflux from lipid vesicles and cells, Defluorination of PFAS by Acidimicrobium sp. A6 and Potential Applications for Remediation, and more.
Additional chapters cover Computational approaches to investigate F- binding, selectivity and transport in F- exporters, Defluorination as a key trait to gauge the biodegradability of fluorinated pollutants: challenges and opportunities, Patch-Clamp Electrophysiology of Fluoride Channels in Saccharomyces cerevisiae, and Synthesis of fluorophenylalanine-encoded proteins in HEK cells.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 - p. p21 9. Fluoride detection, transport and resistance - p. p21 10. Chapter One: Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans - p. 4 11. Chapter Two: NMR methods to detect fluoride binding and transport by membrane proteins - p. 26 12. Chapter Three: Lanthanum-fluoride electrode-based methods to monitor fluoride transport in cells and reconstituted lipid vesicles - p. 44 13. Chapter Four: Microwell fluoride assay screening for enzymatic defluorination - p. 66 14. Chapter Five: Direct fluoride monitoring using a fluorogenic RNA-based biosensor - p. 86 15. Chapter Six: Computational approaches to investigate fluoride binding, selectivity and transport across the membrane - p. 110 16. Chapter Seven: Measurement and analysis of microbial fluoride resistance in dental biofilm models - p. 157 17. Section 2 - p. 172 18. Section 2 Enzymatic fluorination - p. 172 19. Chapter Eight: Preparation, assay, and application of 4-fluorothreonine transaldolase from Streptomyces sp. MA37 for β-hydroxyl amino acid derivatives - p. 178 20. Chapter Nine: Synthesis of fluorinated amino acids by low-specificity, promiscuous aldolases coupled to in situ fluorodonor generation - p. 201 21. Chapter Ten: Radical fluorine transfer catalysed by an engineered nonheme iron enzyme - p. 232 22. Section 3 - p. 246 23. Section 3 Biodegradation of organofluorine compounds - p. 246 24. Chapter Eleven: Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans - p. 253 25. Chapter Twelve: Defluorination of PFAS by Acidimicrobium sp. strain A6 and potential applications for remediation - p. 288 26. Chapter Thirteen: Defluorination as the key trait to gauge the biodegradability of fluorinated pollutants in environmental microbial communities - p. 322 27. Section 4 - p. 336 28. Section 4 Fluorine as a biochemical probe - p. 336 29. Chapter Fourteen: Expression and purification of fluorinated proteins from mammalian suspension culture - p. 342
