Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques.
Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.
1. Chapter 1. Introduction to recombinant protein expression and purification - p. 3 2. Section B: Recombinant protein expression in different expression hosts - p. 30 3. Recombinant protein expression in Escherichia coli - p. 39 4. Chapter 3. Recombinant protein expression in yeast - p. 57 5. Chapter 4. Recombinant protein expression in mammalian cells - p. 79 6. Chlamydomonas reinhardtii for the production of recombinant proteins: current knowledge and perspectives - p. 103 7. Chapter 6. Filamentous fungi as cell factories for heterogeneous protein production - p. 143 8. Section C: Purification strategies and characterization of recombinant proteins - p. 161 9. Chapter 7. Current scenario of recombinant proteins: extraction, purification, concentration, and storage - p. 173 10. Chapter 8. Protein purification: chromatographic and nonchromatographic techniques for purifying recombinant proteins - p. 191 11. Chapter 9. An overview of the contamination sources in recombinant proteins and their characterization - p. 217 12. Chapter 10. Analytical methods for evaluating and characterizing recombinant proteins - p. 243 13. Index - p. 269
Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques.
Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.
1. Chapter 1. Introduction to recombinant protein expression and purification - p. 3 2. Section B: Recombinant protein expression in different expression hosts - p. 30 3. Recombinant protein expression in Escherichia coli - p. 39 4. Chapter 3. Recombinant protein expression in yeast - p. 57 5. Chapter 4. Recombinant protein expression in mammalian cells - p. 79 6. Chlamydomonas reinhardtii for the production of recombinant proteins: current knowledge and perspectives - p. 103 7. Chapter 6. Filamentous fungi as cell factories for heterogeneous protein production - p. 143 8. Section C: Purification strategies and characterization of recombinant proteins - p. 161 9. Chapter 7. Current scenario of recombinant proteins: extraction, purification, concentration, and storage - p. 173 10. Chapter 8. Protein purification: chromatographic and nonchromatographic techniques for purifying recombinant proteins - p. 191 11. Chapter 9. An overview of the contamination sources in recombinant proteins and their characterization - p. 217 12. Chapter 10. Analytical methods for evaluating and characterizing recombinant proteins - p. 243 13. Index - p. 269
