Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. G4 and i-motif Biology - p. iii 8. Chapter One: Purification and biochemical characterization of the G4 resolvase and DNA helicase FANCJ - p. 1 9. Chapter Two: In-gel staining methods of G4 DNA and RNA structures - p. 29 10. Chapter Three: A beginner’s handbook to identify and characterize i-motif DNA - p. 45 11. Chapter Four: Single-molecule observation of G-quadruplex and R-loop formation induced by transcription - p. 71 12. Chapter Five: Advancing the study of protein-G4 interactions in DNA repair: Insights from biolayer interferometry - p. 89 13. Chapter Six: Immunofluorescence microscopy of G-quadruplexes and R-loops - p. 103 14. Chapter Seven: Observing G4 formation and its resolution by Pif1 in real time by manipulation under magnetic tweezers - p. 119 15. Chapter Eight: In vivo detection of DNA secondary structures using permanganate/S1 footprinting with direct adapter ligation and sequencing (PDAL-Seq) - p. 159 16. Chapter Nine: Production of the anti-G-quadruplex antibody BG4 for efficient genome-wide analyses: From plasmid quality control to antibody validation - p. 193 17. Chapter Ten: Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures - p. 221 18. Chapter Eleven: Potentiometric titrations to study ligand interactions with DNA i-motifs - p. 233 19. Chapter Twelve: Identification of bona fide RNA G-quadruplex binding proteins - p. 255
Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. G4 and i-motif Biology - p. iii 8. Chapter One: Purification and biochemical characterization of the G4 resolvase and DNA helicase FANCJ - p. 1 9. Chapter Two: In-gel staining methods of G4 DNA and RNA structures - p. 29 10. Chapter Three: A beginner’s handbook to identify and characterize i-motif DNA - p. 45 11. Chapter Four: Single-molecule observation of G-quadruplex and R-loop formation induced by transcription - p. 71 12. Chapter Five: Advancing the study of protein-G4 interactions in DNA repair: Insights from biolayer interferometry - p. 89 13. Chapter Six: Immunofluorescence microscopy of G-quadruplexes and R-loops - p. 103 14. Chapter Seven: Observing G4 formation and its resolution by Pif1 in real time by manipulation under magnetic tweezers - p. 119 15. Chapter Eight: In vivo detection of DNA secondary structures using permanganate/S1 footprinting with direct adapter ligation and sequencing (PDAL-Seq) - p. 159 16. Chapter Nine: Production of the anti-G-quadruplex antibody BG4 for efficient genome-wide analyses: From plasmid quality control to antibody validation - p. 193 17. Chapter Ten: Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures - p. 221 18. Chapter Eleven: Potentiometric titrations to study ligand interactions with DNA i-motifs - p. 233 19. Chapter Twelve: Identification of bona fide RNA G-quadruplex binding proteins - p. 255
