Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals.
In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource.
1. 1. Introduction - p. 22 2. 2. Institutional Factors - p. 22 3. 3. Isolator Selection - p. 27 4. 4. Sterilization - p. 32 5. 5. Operation - p. 38 6. 6. Resources - p. 52 7. Glossary - p. 56 8. Chapter 3. Procedures for Rodents - p. 65 9. 1. Introduction - p. 66 10. 2. Background and Acknowledgments - p. 67 11. 3. General Information - p. 68 12. 4. Sterilization and Sterilization Monitoring - p. 71 13. 5. Sterilizing Cylinders - p. 86 14. 6. Water - p. 136 15. 7. Transferring Supplies Into Isolators - p. 141 16. 8. Isolator Setup - p. 183 17. 9. Husbandry - p. 290 18. 10. Microbiological Monitoring - p. 298 19. 11. Records and Maintenance - p. 310 20. 12. Derivation of Germfree Mice - p. 312 21. 13. Establishing Microbiota in Germfree Mice - p. 317 22. 14. Alternatives to Isolators - p. 318 23. 15. Shipping and Receiving Mice - p. 320 24. 16. Materials Lists and Fabrication Diagrams - p. 323 25. Chapter 4. Developing a Business Plan for Your Gnotobiotics Program - p. 335 26. Chapter 5. Gnotobiotic Pigs: From Establishing Facility to Modeling Human Infectious Diseases - p. 349 27. Chapter 6. Gnotobiotic Fish as Models to Study Host–Microbe Interactions - p. 369 28. Chapter 7. Use of Gnotobiotic Mice in the Study of Metabolic Syndrome - p. 385 29. Chapter 8. Gnotobiotics and Inflammatory Bowel Disease - p. 391 30. 1. Introduction - p. 391 31. 2. Cytokines and Cytokine Signaling - p. 394 32. 3. T-Cell Receptors and MHC - p. 396 33. 4. Other Mutants - p. 397 34. 5. T-Cell Transfer - p. 399 35. 6. Inbred Strains - p. 401 36. 7. Conclusion - p. 401 37. Index - p. 411
Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals.
In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource.
1. 1. Introduction - p. 22 2. 2. Institutional Factors - p. 22 3. 3. Isolator Selection - p. 27 4. 4. Sterilization - p. 32 5. 5. Operation - p. 38 6. 6. Resources - p. 52 7. Glossary - p. 56 8. Chapter 3. Procedures for Rodents - p. 65 9. 1. Introduction - p. 66 10. 2. Background and Acknowledgments - p. 67 11. 3. General Information - p. 68 12. 4. Sterilization and Sterilization Monitoring - p. 71 13. 5. Sterilizing Cylinders - p. 86 14. 6. Water - p. 136 15. 7. Transferring Supplies Into Isolators - p. 141 16. 8. Isolator Setup - p. 183 17. 9. Husbandry - p. 290 18. 10. Microbiological Monitoring - p. 298 19. 11. Records and Maintenance - p. 310 20. 12. Derivation of Germfree Mice - p. 312 21. 13. Establishing Microbiota in Germfree Mice - p. 317 22. 14. Alternatives to Isolators - p. 318 23. 15. Shipping and Receiving Mice - p. 320 24. 16. Materials Lists and Fabrication Diagrams - p. 323 25. Chapter 4. Developing a Business Plan for Your Gnotobiotics Program - p. 335 26. Chapter 5. Gnotobiotic Pigs: From Establishing Facility to Modeling Human Infectious Diseases - p. 349 27. Chapter 6. Gnotobiotic Fish as Models to Study Host–Microbe Interactions - p. 369 28. Chapter 7. Use of Gnotobiotic Mice in the Study of Metabolic Syndrome - p. 385 29. Chapter 8. Gnotobiotics and Inflammatory Bowel Disease - p. 391 30. 1. Introduction - p. 391 31. 2. Cytokines and Cytokine Signaling - p. 394 32. 3. T-Cell Receptors and MHC - p. 396 33. 4. Other Mutants - p. 397 34. 5. T-Cell Transfer - p. 399 35. 6. Inbred Strains - p. 401 36. 7. Conclusion - p. 401 37. Index - p. 411
