Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Chapter One: Monitoring helicase-catalyzed unwinding of multiple duplexes simultaneously - p. 1 8. Chapter Two: Alignment of helicases on single-stranded DNA increases activity - p. 29 9. Chapter Three: Use of an unnatural amino acid to map helicase/DNA interfaces via photoactivated crosslinking - p. 55 10. Chapter Four: Quantitative methods to study helicase, DNA polymerase, and exonuclease coupling during DNA replication - p. 75 11. Chapter Five: Assembling bacteriophage T7 leading-strand replisome for structural investigation - p. 103 12. Chapter Six: In vivo fluorescent TUNEL detection of single stranded DNA gaps and breaks induced by dnaB helicase mutants in Escherichia coli - p. 125 13. Chapter Seven: Biochemical methods to monitor loading and activation of hexameric helicases - p. 143 14. Chapter Eight: DNA fiber analyses to study functional importance of helicases and associated factors during replication stress - p. 153 15. Chapter Nine: Optimizing CMG helicase and CMG-dependent replication assays by designing DNA fork substrates and choosing nucleotide analogues for helicase preloading - p. 173 16. Chapter Ten: ReconSil: An electron microscopy toolbox to study helicase function at an origin of replication - p. 203 17. Chapter Eleven: CMG helicase activity on G4-containing DNA templates - p. 233 18. Chapter Twelve: Detecting G4 unwinding - p. 261 19. Chapter Thirteen: Helicase mediated vectorial folding of telomere G-quadruplex - p. 283 20. Chapter Fourteen: Production of long linear DNA substrates with site-specific chemical lesions for single-molecule replisome studies - p. 302 21. Chapter Fifteen: Replication fork collapse in vitro using Xenopus egg extracts - p. 317 22. Chapter Sixteen: Measuring the contributions of helicases to break-induced replication - p. 339 23. Chapter Seventeen: A RADAR method to measure DNA topoisomerase covalent complexes - p. 369
Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Chapter One: Monitoring helicase-catalyzed unwinding of multiple duplexes simultaneously - p. 1 8. Chapter Two: Alignment of helicases on single-stranded DNA increases activity - p. 29 9. Chapter Three: Use of an unnatural amino acid to map helicase/DNA interfaces via photoactivated crosslinking - p. 55 10. Chapter Four: Quantitative methods to study helicase, DNA polymerase, and exonuclease coupling during DNA replication - p. 75 11. Chapter Five: Assembling bacteriophage T7 leading-strand replisome for structural investigation - p. 103 12. Chapter Six: In vivo fluorescent TUNEL detection of single stranded DNA gaps and breaks induced by dnaB helicase mutants in Escherichia coli - p. 125 13. Chapter Seven: Biochemical methods to monitor loading and activation of hexameric helicases - p. 143 14. Chapter Eight: DNA fiber analyses to study functional importance of helicases and associated factors during replication stress - p. 153 15. Chapter Nine: Optimizing CMG helicase and CMG-dependent replication assays by designing DNA fork substrates and choosing nucleotide analogues for helicase preloading - p. 173 16. Chapter Ten: ReconSil: An electron microscopy toolbox to study helicase function at an origin of replication - p. 203 17. Chapter Eleven: CMG helicase activity on G4-containing DNA templates - p. 233 18. Chapter Twelve: Detecting G4 unwinding - p. 261 19. Chapter Thirteen: Helicase mediated vectorial folding of telomere G-quadruplex - p. 283 20. Chapter Fourteen: Production of long linear DNA substrates with site-specific chemical lesions for single-molecule replisome studies - p. 302 21. Chapter Fifteen: Replication fork collapse in vitro using Xenopus egg extracts - p. 317 22. Chapter Sixteen: Measuring the contributions of helicases to break-induced replication - p. 339 23. Chapter Seventeen: A RADAR method to measure DNA topoisomerase covalent complexes - p. 369
