Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Chapter One: Fluorescence approaches for biochemical analysis of ATP-dependent chromatin remodeling enzymes - p. 1 9. Chapter Two: Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond - p. 19 10. Chapter Three: Monitoring functional RNA binding of RNA-dependent ATPase enzymes such as SF2 helicases using RNA dependent ATPase assays: A RIG-I case study - p. 39 11. Chapter Four: Measurement of ATP utilization in RNA unwinding and RNA chaperone activities by DEAD-box helicase proteins - p. 53 12. Chapter Five: In vitro characterization of Dhr1 from Saccharomyces cerevisiae - p. 77 13. Chapter Six: Translational control by helicases during cellular stress - p. 103 14. Chapter Seven: Lysate and cell-based assays to probe the translational role of RNA helicases - p. 141 15. Chapter Eight: Bulk phase biochemistry of PIF1 and RecQ4 family helicases - p. 179 16. Chapter Nine: Biochemical and single-molecule techniques to study accessory helicase resolution of R-loop proteins at stalled replication forks - p. 191 17. Chapter Ten: Transcriptional regulation by a RecQ helicase - p. 227 18. Chapter Eleven: Analysis of the conformational space and dynamics of RNA helicases by single-molecule FRET in solution and on surfaces - p. 251 19. Chapter Twelve: Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers - p. 311 20. Chapter Thirteen: Novel approaches to study helicases using magnetic tweezers - p. 359 21. Chapter Fourteen: Reconstitution and biochemical characterization of the RNA-guided helicase-nuclease protein Cas3 from type I-A CRISPR–Cas system - p. 405 22. Chapter Fifteen: Purification and characterization of Mtr4 and TRAMP from S. cerevisiae - p. 425 23. Chapter Sixteen: Methods to assess helicase and translocation activities of human nuclear RNA exosome and RNA adaptor complexes - p. 453 24. Chapter Seventeen: Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA - p. 475
Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Chapter One: Fluorescence approaches for biochemical analysis of ATP-dependent chromatin remodeling enzymes - p. 1 9. Chapter Two: Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond - p. 19 10. Chapter Three: Monitoring functional RNA binding of RNA-dependent ATPase enzymes such as SF2 helicases using RNA dependent ATPase assays: A RIG-I case study - p. 39 11. Chapter Four: Measurement of ATP utilization in RNA unwinding and RNA chaperone activities by DEAD-box helicase proteins - p. 53 12. Chapter Five: In vitro characterization of Dhr1 from Saccharomyces cerevisiae - p. 77 13. Chapter Six: Translational control by helicases during cellular stress - p. 103 14. Chapter Seven: Lysate and cell-based assays to probe the translational role of RNA helicases - p. 141 15. Chapter Eight: Bulk phase biochemistry of PIF1 and RecQ4 family helicases - p. 179 16. Chapter Nine: Biochemical and single-molecule techniques to study accessory helicase resolution of R-loop proteins at stalled replication forks - p. 191 17. Chapter Ten: Transcriptional regulation by a RecQ helicase - p. 227 18. Chapter Eleven: Analysis of the conformational space and dynamics of RNA helicases by single-molecule FRET in solution and on surfaces - p. 251 19. Chapter Twelve: Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers - p. 311 20. Chapter Thirteen: Novel approaches to study helicases using magnetic tweezers - p. 359 21. Chapter Fourteen: Reconstitution and biochemical characterization of the RNA-guided helicase-nuclease protein Cas3 from type I-A CRISPR–Cas system - p. 405 22. Chapter Fifteen: Purification and characterization of Mtr4 and TRAMP from S. cerevisiae - p. 425 23. Chapter Sixteen: Methods to assess helicase and translocation activities of human nuclear RNA exosome and RNA adaptor complexes - p. 453 24. Chapter Seventeen: Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA - p. 475
