Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Immuno-oncology and immunotherapy - Part A - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. ii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One Assessment of translation rate in leukemic cells and immune cells of the microenvironment by OPP protein synthesis assay - p. 1 9. Chapter Two Generation of transmitochondrial cybrids in cancer cells - p. 24 10. Chapter Three Enzyme-linked ImmunoSpot (ELISpot) assay to quantify peptide-specific IFN-γ production by splenocytes in a mouse tumor model after radiation therapy - p. 41 11. Chapter Four Tumor slice culture system for ex vivo immunotherapy studies - p. 55 12. Chapter Five Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells - p. 72 13. Chapter Six Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors - p. 86 14. Chapter Seven Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids - p. 98 15. Chapter Eight Characterization of Natural Killer cells infiltrating irradiated murine tumors through flow cytometry - p. 118 16. Chapter Nine Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation - p. 135 17. Chapter Ten Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line - p. 153 18. Chapter Eleven Optimization and intra-assay validation of a multiparametric flow cytometric test for monitoring circulating TREGs - p. 170 19. Chapter Twelve Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow - p. 189
Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Immuno-oncology and immunotherapy - Part A - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. ii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One Assessment of translation rate in leukemic cells and immune cells of the microenvironment by OPP protein synthesis assay - p. 1 9. Chapter Two Generation of transmitochondrial cybrids in cancer cells - p. 24 10. Chapter Three Enzyme-linked ImmunoSpot (ELISpot) assay to quantify peptide-specific IFN-γ production by splenocytes in a mouse tumor model after radiation therapy - p. 41 11. Chapter Four Tumor slice culture system for ex vivo immunotherapy studies - p. 55 12. Chapter Five Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells - p. 72 13. Chapter Six Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors - p. 86 14. Chapter Seven Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids - p. 98 15. Chapter Eight Characterization of Natural Killer cells infiltrating irradiated murine tumors through flow cytometry - p. 118 16. Chapter Nine Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation - p. 135 17. Chapter Ten Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line - p. 153 18. Chapter Eleven Optimization and intra-assay validation of a multiparametric flow cytometric test for monitoring circulating TREGs - p. 170 19. Chapter Twelve Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow - p. 189
