Immuno-oncology and immunotherapy, Part B, Volume 191 in the Methods in Cell Biology series, highlights new advances, with this volume presenting chapters on a variety of topics, including Multiparametric flow cytometry study of human MDSCs, In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance, Machine learning approach to assess brain metastatic burden in preclinical models, Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death, 3D agar colony inhibition assays to functionally evaluate HER2-targeting antibodies against human cancer cells elicited by vaccination in mice, and much more.
Additional sections cover Spatial immunophenotyping of FFPE tissues by imaging mass cytometry, Processing human colon cancer specimens for in vitro cytotoxicity assays, A xenograft model of metastatic colon cancer, A preclinical model of hepatic metastasis to instruct effective treatment modalities, Myeloid-derived suppressor cells: Identification and function, Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer, and Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling - p. 1 8. Chapter Two: In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance - p. 11 9. Chapter Three: Machine learning approach to assess brain metastatic burden in preclinical models - p. 27 10. Chapter Four: Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death - p. 51 11. Chapter Five: Functional evaluation of circulating anti-cancer antibodies with a 3D tumor cell growth inhibition assay - p. 75 12. Chapter Six: Spatial immunophenotyping of FFPE tissues by imaging mass cytometry - p. 87 13. Chapter Seven: Processing human colon cancer specimens for in vitro cytotoxicity assays - p. 105 14. Chapter Eight: An orthotopic metastatic xenograft model of colorectal cancer - p. 119 15. Chapter Nine: A preclinical mouse model of hepatic metastasis to instruct effective treatment modalities - p. 133 16. Chapter Ten: Myeloid-derived suppressor cells: Identification and function - p. 151 17. Chapter Eleven: Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer - p. 171 18. Chapter Twelve: Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse - p. 203
Immuno-oncology and immunotherapy, Part B, Volume 191 in the Methods in Cell Biology series, highlights new advances, with this volume presenting chapters on a variety of topics, including Multiparametric flow cytometry study of human MDSCs, In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance, Machine learning approach to assess brain metastatic burden in preclinical models, Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death, 3D agar colony inhibition assays to functionally evaluate HER2-targeting antibodies against human cancer cells elicited by vaccination in mice, and much more.
Additional sections cover Spatial immunophenotyping of FFPE tissues by imaging mass cytometry, Processing human colon cancer specimens for in vitro cytotoxicity assays, A xenograft model of metastatic colon cancer, A preclinical model of hepatic metastasis to instruct effective treatment modalities, Myeloid-derived suppressor cells: Identification and function, Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer, and Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling - p. 1 8. Chapter Two: In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance - p. 11 9. Chapter Three: Machine learning approach to assess brain metastatic burden in preclinical models - p. 27 10. Chapter Four: Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death - p. 51 11. Chapter Five: Functional evaluation of circulating anti-cancer antibodies with a 3D tumor cell growth inhibition assay - p. 75 12. Chapter Six: Spatial immunophenotyping of FFPE tissues by imaging mass cytometry - p. 87 13. Chapter Seven: Processing human colon cancer specimens for in vitro cytotoxicity assays - p. 105 14. Chapter Eight: An orthotopic metastatic xenograft model of colorectal cancer - p. 119 15. Chapter Nine: A preclinical mouse model of hepatic metastasis to instruct effective treatment modalities - p. 133 16. Chapter Ten: Myeloid-derived suppressor cells: Identification and function - p. 151 17. Chapter Eleven: Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer - p. 171 18. Chapter Twelve: Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse - p. 203
