Immuno-oncology and immunotherapy, Part F, Volume 201 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Generation and functional evaluation of STAb-T cells - p. 1 9. Chapter Two: A syngeneic orthotopic mouse model of metastatic colorectal cancer - p. 23 10. Chapter Three: High-parameter T-cell spectral phospho-flow-cytometry - p. 39 11. Chapter Four: Measuring T-cell/CAR T-cell bystander tumor killing in vitro and in vivo - p. 55 12. Chapter Five: Quantification and functional assessment of antigen(-) tumor cell variants - p. 77 13. Chapter Six: Mouse and human T cell Cas9-RNP/nucleofection-mediated gene-editing - p. 91 14. Chapter Seven: Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics - p. 109 15. Chapter Eight: Integrative analysis of lung multiparametric flow cytometry data to study immune cell dynamics in tumors - p. 121 16. Chapter Nine: Multiparametric staining - combined application of immunofluorescence and in-situ hybridization - p. 155 17. Chapter ten: Extracellular matrix and morphology assessment method on live 3D spheroids of thyroid carcinoma - p. 169 18. Chapter Eleven: Hema-CRISPR: A clone-based protocol for effective genetic editing of hematopoietic stem cells - p. 185 19. Chapter Twelve: Patient-derived models of tumor-immune cell interactions - p. 195 20. Chapter Thirteen: In vitro generation of human CD14+ MDSC-like cells from normal human monocytes - p. 219 21. Chapter Fourteen: Extracellular vesicles isolation from human maternal fluids - p. 231 22. Chapter Fifteen: Finding a needle in a haystack: Measures of circulating tumor DNA (ctDNA) to guide treatment decisions in oncology - p. 237
Immuno-oncology and immunotherapy, Part F, Volume 201 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Generation and functional evaluation of STAb-T cells - p. 1 9. Chapter Two: A syngeneic orthotopic mouse model of metastatic colorectal cancer - p. 23 10. Chapter Three: High-parameter T-cell spectral phospho-flow-cytometry - p. 39 11. Chapter Four: Measuring T-cell/CAR T-cell bystander tumor killing in vitro and in vivo - p. 55 12. Chapter Five: Quantification and functional assessment of antigen(-) tumor cell variants - p. 77 13. Chapter Six: Mouse and human T cell Cas9-RNP/nucleofection-mediated gene-editing - p. 91 14. Chapter Seven: Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics - p. 109 15. Chapter Eight: Integrative analysis of lung multiparametric flow cytometry data to study immune cell dynamics in tumors - p. 121 16. Chapter Nine: Multiparametric staining - combined application of immunofluorescence and in-situ hybridization - p. 155 17. Chapter ten: Extracellular matrix and morphology assessment method on live 3D spheroids of thyroid carcinoma - p. 169 18. Chapter Eleven: Hema-CRISPR: A clone-based protocol for effective genetic editing of hematopoietic stem cells - p. 185 19. Chapter Twelve: Patient-derived models of tumor-immune cell interactions - p. 195 20. Chapter Thirteen: In vitro generation of human CD14+ MDSC-like cells from normal human monocytes - p. 219 21. Chapter Fourteen: Extracellular vesicles isolation from human maternal fluids - p. 231 22. Chapter Fifteen: Finding a needle in a haystack: Measures of circulating tumor DNA (ctDNA) to guide treatment decisions in oncology - p. 237
