Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques.
This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. List of contributors - p. xv 7. Section 1: Fundamentals and practice of thin-layer chromatography - p. xvii 8. Chapter 1. Milestones, core concepts, and contrasts - p. 3 9. Chapter 2. High-performance precoated stationary phases - p. 27 10. Chapter 3. Solvent selection and method development for thin-layer chromatography - p. 47 11. Chapter 4. Automated multiple development - p. 81 12. Chapter 5. Instrument platforms for high-performance thin-layer chromatography - p. 111 13. Chapter 6. Theory and instrumentation for in situ detection - p. 143 14. Chapter 7. Advanced spectroscopic detectors for identification and quantification: infrared, Raman, and nuclear magnetic resonance - p. 165 15. Chapter 8. Advanced spectroscopic detectors for identification and quantification: mass spectrometry - p. 181 16. Chapter 9. Staining and derivatization techniques for visualization in planar chromatography - p. 213 17. Chapter 10. Effects-directed detection: cell-based assays - p. 259 18. Chapter 11. Effect-directed detection: chemical and enzyme-based assays - p. 297 19. Chapter 12. Validation of thin-layer chromatographic methods - p. 325 20. Chapter 13. Data analysis tools in thin-layer chromatography - p. 361 21. Chapter 14. Microfluidic and small-scale planar separation systems - p. 395 22. Section 2: Applications of thin-layer chromatography - p. 409 23. Chapter 15. Application of thin-layer chromatography to the analysis of saccharides - p. 413 24. Chapter 16. Applications of thin-layer chromatography to the analysis of lipids - p. 437 25. Chapter 17. Applications in food analysis - p. 473 26. Chapter 18. Applications of thin-layer chromatography in environmental analysis - p. 493 27. Chapter 19. Applications of thin-layer chromatography in the pharmaceutical industry - p. 519 28. Chapter 20. Applications of thin-layer chromatography in the quality control of botanicals - p. 575 29. Chapter 21. Applications of thin-layer chromatography to the quality control of dietary supplements - p. 615 30. Index - p. 665
Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques.
This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. List of contributors - p. xv 7. Section 1: Fundamentals and practice of thin-layer chromatography - p. xvii 8. Chapter 1. Milestones, core concepts, and contrasts - p. 3 9. Chapter 2. High-performance precoated stationary phases - p. 27 10. Chapter 3. Solvent selection and method development for thin-layer chromatography - p. 47 11. Chapter 4. Automated multiple development - p. 81 12. Chapter 5. Instrument platforms for high-performance thin-layer chromatography - p. 111 13. Chapter 6. Theory and instrumentation for in situ detection - p. 143 14. Chapter 7. Advanced spectroscopic detectors for identification and quantification: infrared, Raman, and nuclear magnetic resonance - p. 165 15. Chapter 8. Advanced spectroscopic detectors for identification and quantification: mass spectrometry - p. 181 16. Chapter 9. Staining and derivatization techniques for visualization in planar chromatography - p. 213 17. Chapter 10. Effects-directed detection: cell-based assays - p. 259 18. Chapter 11. Effect-directed detection: chemical and enzyme-based assays - p. 297 19. Chapter 12. Validation of thin-layer chromatographic methods - p. 325 20. Chapter 13. Data analysis tools in thin-layer chromatography - p. 361 21. Chapter 14. Microfluidic and small-scale planar separation systems - p. 395 22. Section 2: Applications of thin-layer chromatography - p. 409 23. Chapter 15. Application of thin-layer chromatography to the analysis of saccharides - p. 413 24. Chapter 16. Applications of thin-layer chromatography to the analysis of lipids - p. 437 25. Chapter 17. Applications in food analysis - p. 473 26. Chapter 18. Applications of thin-layer chromatography in environmental analysis - p. 493 27. Chapter 19. Applications of thin-layer chromatography in the pharmaceutical industry - p. 519 28. Chapter 20. Applications of thin-layer chromatography in the quality control of botanicals - p. 575 29. Chapter 21. Applications of thin-layer chromatography to the quality control of dietary supplements - p. 615 30. Index - p. 665