Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Biochemical analysis of protein–protein interfaces underlying the regulation of bacterial secretion systems - p. 1 8. Chapter Two: Quantitative structural proteomics in living cells by covalent protein painting - p. 33 9. Chapter Three: Probing protein misfolding and dissociation with an infrared free-electron laser - p. 65 10. Chapter Four: Optimized protocols for the characterization of Cas12a activities - p. 97 11. Chapter Five: Proximity Labeling and Proteomics: Get to Know Neighbors - p. 131 12. Chapter Six: Looking at LPMO reactions through the lens of the HRP/Amplex Red assay - p. 171 13. Chapter Seven: Bioinformatic prediction and experimental validation of RiPP recognition elements - p. 191 14. Chapter Eight: The preparation of recombinant arginyltransferase 1 (ATE1) for biophysical characterization - p. 235 15. Chapter Nine: Testing anti-cancer drugs with holographic incoherent-light-source quantitative phase imaging - p. 255 16. Chapter Ten: A streamlined process for discovery and characterization of inhibitors against phenylalanyl-tRNA synthetase of Mycobacterium tuberculosis - p. 275 17. Chapter Eleven: Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry - p. 295 18. Chapter Twelve: Generation of nucleotide-linked resins for identification of novel binding proteins - p. 323 19. Chapter Thirteen: Functional assay of light-induced ion-transport by rhodopsins - p. 331 20. Chapter Fourteen: Assay design for analysis of human uracil DNA glycosylase - p. 343 21. Chapter Fifteen: Measurement of kinetic isotope effects on peptide hydroxylation using MALDI-MS - p. 363 22. Chapter Sixteen: Continuous photometric activity assays for lytic polysaccharide monooxygenase—Critical assessment and practical considerations - p. 381
Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Biochemical analysis of protein–protein interfaces underlying the regulation of bacterial secretion systems - p. 1 8. Chapter Two: Quantitative structural proteomics in living cells by covalent protein painting - p. 33 9. Chapter Three: Probing protein misfolding and dissociation with an infrared free-electron laser - p. 65 10. Chapter Four: Optimized protocols for the characterization of Cas12a activities - p. 97 11. Chapter Five: Proximity Labeling and Proteomics: Get to Know Neighbors - p. 131 12. Chapter Six: Looking at LPMO reactions through the lens of the HRP/Amplex Red assay - p. 171 13. Chapter Seven: Bioinformatic prediction and experimental validation of RiPP recognition elements - p. 191 14. Chapter Eight: The preparation of recombinant arginyltransferase 1 (ATE1) for biophysical characterization - p. 235 15. Chapter Nine: Testing anti-cancer drugs with holographic incoherent-light-source quantitative phase imaging - p. 255 16. Chapter Ten: A streamlined process for discovery and characterization of inhibitors against phenylalanyl-tRNA synthetase of Mycobacterium tuberculosis - p. 275 17. Chapter Eleven: Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry - p. 295 18. Chapter Twelve: Generation of nucleotide-linked resins for identification of novel binding proteins - p. 323 19. Chapter Thirteen: Functional assay of light-induced ion-transport by rhodopsins - p. 331 20. Chapter Fourteen: Assay design for analysis of human uracil DNA glycosylase - p. 343 21. Chapter Fifteen: Measurement of kinetic isotope effects on peptide hydroxylation using MALDI-MS - p. 363 22. Chapter Sixteen: Continuous photometric activity assays for lytic polysaccharide monooxygenase—Critical assessment and practical considerations - p. 381
