Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Nanoluciferase-based complementation assays to monitor activation, modulation and signaling of receptor tyrosine kinases (RTKs) - p. 1 9. Chapter Two: Molecular regulation of PLCβ signaling - p. 39 10. Chapter Three: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 1: Chip development, system operation and measurement setup - p. 53 11. Chapter Four: Spectroscopic analysis of the mammalian enzyme cysteine dioxygenase - p. 101 12. Chapter Five: DeGlyPHER: Highly sensitive site-specific analysis of N-linked glycans on proteins - p. 137 13. Chapter Six: Sample preparation for structural mass spectrometry via polyacrylamide gel electrophoresis - p. 187 14. Chapter Seven: High-throughput screening of glycosynthases using azido sugars for oligosaccharides synthesis - p. 211 15. Chapter Eight: Rational design, production and in vitro analysis of photoxenoproteins - p. 247 16. Chapter Nine: Chemical and structural approaches to investigate PTEN function and regulation - p. 297 17. Chapter Ten: Analyzing protein posttranslational modifications using enzyme-catalyzed expressed protein ligation - p. 319 18. Chapter Eleven: Application of hybrid biophysical-biochemical methods to unravel the molecular basis for auto-inhibition and activation of protein tyrosine phosphatase TCPTP/PTPN2 - p. 351 19. Chapter Twelve: Multiplex substrate profiling by mass spectrometry for proteases - p. 384 20. Chapter Thirteen: Protein labeling and crosslinking by covalent aptamers - p. 413 21. Chapter Fourteen: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 2: Data extraction, processing and analysis - p. 429 22. Chapter Fifteen: A streamlined protocol for expression and purification of wild-type β-arrestins - p. 465
Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Nanoluciferase-based complementation assays to monitor activation, modulation and signaling of receptor tyrosine kinases (RTKs) - p. 1 9. Chapter Two: Molecular regulation of PLCβ signaling - p. 39 10. Chapter Three: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 1: Chip development, system operation and measurement setup - p. 53 11. Chapter Four: Spectroscopic analysis of the mammalian enzyme cysteine dioxygenase - p. 101 12. Chapter Five: DeGlyPHER: Highly sensitive site-specific analysis of N-linked glycans on proteins - p. 137 13. Chapter Six: Sample preparation for structural mass spectrometry via polyacrylamide gel electrophoresis - p. 187 14. Chapter Seven: High-throughput screening of glycosynthases using azido sugars for oligosaccharides synthesis - p. 211 15. Chapter Eight: Rational design, production and in vitro analysis of photoxenoproteins - p. 247 16. Chapter Nine: Chemical and structural approaches to investigate PTEN function and regulation - p. 297 17. Chapter Ten: Analyzing protein posttranslational modifications using enzyme-catalyzed expressed protein ligation - p. 319 18. Chapter Eleven: Application of hybrid biophysical-biochemical methods to unravel the molecular basis for auto-inhibition and activation of protein tyrosine phosphatase TCPTP/PTPN2 - p. 351 19. Chapter Twelve: Multiplex substrate profiling by mass spectrometry for proteases - p. 384 20. Chapter Thirteen: Protein labeling and crosslinking by covalent aptamers - p. 413 21. Chapter Fourteen: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 2: Data extraction, processing and analysis - p. 429 22. Chapter Fifteen: A streamlined protocol for expression and purification of wild-type β-arrestins - p. 465
