Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This international handbook, based on a series of workshops carried out for governments in collaboration with ILSI and Co-published in partnership with the Cereals & Grains Association, provides the technical and practical information needed to develop, validate and use detection methods. This useful resource provides readers with the tools necessary to carry out reliable sampling, detection and interpretation of data.
1. Chapter 1. Introduction: genetically modified crops and their detection - p. 1 2. Chapter 2. Seed purity testing and low-level presence - p. 7 3. Chapter 3. Grain supply chain stewardship and testing—import and export - p. 21 4. Chapter 4. Principles of nucleic acid-based detection methods - p. 41 5. Chapter 5. Method validation: DNA-based detection methods - p. 67 6. Chapter 6. Protein-based detection methods - p. 85 7. Chapter 7. Protein methods: antibody-based protein method validation and assay verification - p. 111 8. Chapter 8. Reference materials and working standards - p. 141 9. Chapter 9. Seed and grain sampling - p. 161 10. Chapter 10. Plant and field sampling - p. 177 11. Chapter 11. Testing laboratory design and management - p. 191 12. Chapter 12. International standards and guidelines - p. 215 13. Chapter 13. Analytical strategy and interpretation of results - p. 227 14. Chapter 14. Detection methods for genome-edited crops - p. 253 15. Chapter 15. Future perspectives and challenges - p. 273 16. Index - p. 295
Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This international handbook, based on a series of workshops carried out for governments in collaboration with ILSI and Co-published in partnership with the Cereals & Grains Association, provides the technical and practical information needed to develop, validate and use detection methods. This useful resource provides readers with the tools necessary to carry out reliable sampling, detection and interpretation of data.
1. Chapter 1. Introduction: genetically modified crops and their detection - p. 1 2. Chapter 2. Seed purity testing and low-level presence - p. 7 3. Chapter 3. Grain supply chain stewardship and testing—import and export - p. 21 4. Chapter 4. Principles of nucleic acid-based detection methods - p. 41 5. Chapter 5. Method validation: DNA-based detection methods - p. 67 6. Chapter 6. Protein-based detection methods - p. 85 7. Chapter 7. Protein methods: antibody-based protein method validation and assay verification - p. 111 8. Chapter 8. Reference materials and working standards - p. 141 9. Chapter 9. Seed and grain sampling - p. 161 10. Chapter 10. Plant and field sampling - p. 177 11. Chapter 11. Testing laboratory design and management - p. 191 12. Chapter 12. International standards and guidelines - p. 215 13. Chapter 13. Analytical strategy and interpretation of results - p. 227 14. Chapter 14. Detection methods for genome-edited crops - p. 253 15. Chapter 15. Future perspectives and challenges - p. 273 16. Index - p. 295
