Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation provides methods and techniques of ovarian tissue harvesting and cryopreservation, including instructional videos. This book will benefit a wide audience, guiding infertility specialists, fellows, residents, reproductive surgeons, reproductive endocrinologists, pediatric surgeons, embryologists, infertility nurses and gynecologists. Ovarian cryopreservation and transplantation is rapidly gaining acceptance as a successful and established fertility preservation strategy in cancer patients and beyond. Unlike other fertility preservation strategies, it can be performed in children as well as adults, and can be helpful in restoring natural ovarian function and fertility, hence this is a welcomed resource on the topic.
1. Introduction - p. xxvii 2. Chapter 1. Scientific History of Ovarian Tissue Cryopreservation and Transplantation - p. 1 3. Chapter 2. Animal Models of Ovarian Transplantation - p. 11 4. Chapter 3. Overall Approach to Fertility Preservation and Indications for Ovarian Tissue Harvesting and Cryopreservation - p. 19 5. Chapter 4. Regulatory Aspects of Ovarian Tissue Cryopreservation and Transplantation: How to Set Up a Program in Your Center - p. 29 6. Chapter 5. Ovarian Reserve and Early Follicle Development: Prerequisite Knowledge for Understanding Ovarian Tissue Harvesting for Cryopreservation - p. 37 7. Chapter 6. Methods and Techniques of Ovarian Tissue Harvesting and Preparation Before Cryopreservation - p. 49 8. Chapter 7. Oocyte Retrieval and In Vitro Maturation From the Harvested Tissue Before Cryopreservation - p. 61 9. Chapter 8. A Primer on Cryobiology and Cryoprotectants for Ovarian Tissue Freezing - p. 67 10. Chapter 9. Methods of Ovarian Tissue Cryopreservation: Slow Freezing - p. 89 11. Chapter 10. Methods of Ovarian Tissue Cryopreservation: Vitrification - p. 99 12. Chapter 11. Preoperative Evaluation and Preparation for Ovarian Tissue Transplant Surgery - p. 109 13. Chapter 12. Assessment of the Risk of Cancer Cell Reintroduction Prior to Ovarian Tissue Autotransplantation - p. 117 14. Chapter 13. Ovarian Cryopreservation for Transplantation With Prepubertal and Adolescent Girls - p. 127 15. Chapter 14. Surgical Approach to Orthotopic Ovarian Tissue Transplantation - p. 143 16. Chapter 15. Surgical Approach to Laparoscopic and Robot-Assisted Ovarian Tissue Transplantation - p. 157 17. Chapter 16. Surgical Approach to Heterotopic Ovarian Tissue Transplantation - p. 169 18. Chapter 17. Approach to Follow Up After Ovarian Transplantation - p. 177 19. Chapter 18. Approach to Ovarian Stimulation and In Vitro Fertilization in Patients With Transplanted Ovarian Tissue - p. 185 20. Chapter 19. Ovarian Tissue Cryopreservation for Delaying Childbearing and Menopause - p. 193 21. Chapter 20. In Vitro Growth and Maturation of Human Primordial Follicles From Cryopreserved Tissue - p. 203 22. Chapter 21. Psychosocial and Ethical Aspects of Ovarian Tissue Cryopreservation in Children and Adults - p. 213 23. Chapter 22. Future Aspects of Ovarian Cryopreservation and Transplantation - p. 221 24. Appendix1. Instructional Videos for Ovarian Tissue Harvesting, Cryopreservation, and Patient Counseling - p. 229 25. Appendix2. Materials, Equipment, and Protocols for Ovarian Tissue Cryopreservation and Thawing - p. 233 26. Appendix3. Materials, Equipment, and Operating Room Set-Up for Ovarian Tissue Transplantation - p. 239 27. Index - p. 243 28. Memoriam - p. 247
Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation provides methods and techniques of ovarian tissue harvesting and cryopreservation, including instructional videos. This book will benefit a wide audience, guiding infertility specialists, fellows, residents, reproductive surgeons, reproductive endocrinologists, pediatric surgeons, embryologists, infertility nurses and gynecologists. Ovarian cryopreservation and transplantation is rapidly gaining acceptance as a successful and established fertility preservation strategy in cancer patients and beyond. Unlike other fertility preservation strategies, it can be performed in children as well as adults, and can be helpful in restoring natural ovarian function and fertility, hence this is a welcomed resource on the topic.
1. Introduction - p. xxvii 2. Chapter 1. Scientific History of Ovarian Tissue Cryopreservation and Transplantation - p. 1 3. Chapter 2. Animal Models of Ovarian Transplantation - p. 11 4. Chapter 3. Overall Approach to Fertility Preservation and Indications for Ovarian Tissue Harvesting and Cryopreservation - p. 19 5. Chapter 4. Regulatory Aspects of Ovarian Tissue Cryopreservation and Transplantation: How to Set Up a Program in Your Center - p. 29 6. Chapter 5. Ovarian Reserve and Early Follicle Development: Prerequisite Knowledge for Understanding Ovarian Tissue Harvesting for Cryopreservation - p. 37 7. Chapter 6. Methods and Techniques of Ovarian Tissue Harvesting and Preparation Before Cryopreservation - p. 49 8. Chapter 7. Oocyte Retrieval and In Vitro Maturation From the Harvested Tissue Before Cryopreservation - p. 61 9. Chapter 8. A Primer on Cryobiology and Cryoprotectants for Ovarian Tissue Freezing - p. 67 10. Chapter 9. Methods of Ovarian Tissue Cryopreservation: Slow Freezing - p. 89 11. Chapter 10. Methods of Ovarian Tissue Cryopreservation: Vitrification - p. 99 12. Chapter 11. Preoperative Evaluation and Preparation for Ovarian Tissue Transplant Surgery - p. 109 13. Chapter 12. Assessment of the Risk of Cancer Cell Reintroduction Prior to Ovarian Tissue Autotransplantation - p. 117 14. Chapter 13. Ovarian Cryopreservation for Transplantation With Prepubertal and Adolescent Girls - p. 127 15. Chapter 14. Surgical Approach to Orthotopic Ovarian Tissue Transplantation - p. 143 16. Chapter 15. Surgical Approach to Laparoscopic and Robot-Assisted Ovarian Tissue Transplantation - p. 157 17. Chapter 16. Surgical Approach to Heterotopic Ovarian Tissue Transplantation - p. 169 18. Chapter 17. Approach to Follow Up After Ovarian Transplantation - p. 177 19. Chapter 18. Approach to Ovarian Stimulation and In Vitro Fertilization in Patients With Transplanted Ovarian Tissue - p. 185 20. Chapter 19. Ovarian Tissue Cryopreservation for Delaying Childbearing and Menopause - p. 193 21. Chapter 20. In Vitro Growth and Maturation of Human Primordial Follicles From Cryopreserved Tissue - p. 203 22. Chapter 21. Psychosocial and Ethical Aspects of Ovarian Tissue Cryopreservation in Children and Adults - p. 213 23. Chapter 22. Future Aspects of Ovarian Cryopreservation and Transplantation - p. 221 24. Appendix1. Instructional Videos for Ovarian Tissue Harvesting, Cryopreservation, and Patient Counseling - p. 229 25. Appendix2. Materials, Equipment, and Protocols for Ovarian Tissue Cryopreservation and Thawing - p. 233 26. Appendix3. Materials, Equipment, and Operating Room Set-Up for Ovarian Tissue Transplantation - p. 239 27. Index - p. 243 28. Memoriam - p. 247
