Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xv 8. Chapter One: Correlating ion channel structure and function - p. 3 9. Chapter Two: TRPV3 expression and purification for structure determination by Cryo-EM - p. 31 10. Chapter Three: Methods to study phosphoinositide regulation of ion channels - p. 49 11. Chapter Four: Recombinant expression and purification of pentameric ligand-gated ion channels for Cryo-EM structural studies - p. 81 12. Chapter Five: Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography - p. 105 13. Chapter Six: Structural dynamics of channels and transporters by high-speed atomic force microscopy - p. 130 14. Chapter Seven: Crosslinking glutamate receptor ion channels - p. 161 15. Chapter Eight: Single molecule FRET methodology for investigating glutamate receptors - p. 193 16. Chapter Nine: Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues - p. 213 17. Chapter Ten: A quantitative flux assay for the study of reconstituted Cl− channels and transporters - p. 243 18. Chapter Eleven: Ion channel reconstitution in lipid bilayers - p. 273 19. Chapter Twelve: Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers - p. 293 20. Chapter Thirteen: The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture - p. 321
Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xv 8. Chapter One: Correlating ion channel structure and function - p. 3 9. Chapter Two: TRPV3 expression and purification for structure determination by Cryo-EM - p. 31 10. Chapter Three: Methods to study phosphoinositide regulation of ion channels - p. 49 11. Chapter Four: Recombinant expression and purification of pentameric ligand-gated ion channels for Cryo-EM structural studies - p. 81 12. Chapter Five: Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography - p. 105 13. Chapter Six: Structural dynamics of channels and transporters by high-speed atomic force microscopy - p. 130 14. Chapter Seven: Crosslinking glutamate receptor ion channels - p. 161 15. Chapter Eight: Single molecule FRET methodology for investigating glutamate receptors - p. 193 16. Chapter Nine: Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues - p. 213 17. Chapter Ten: A quantitative flux assay for the study of reconstituted Cl− channels and transporters - p. 243 18. Chapter Eleven: Ion channel reconstitution in lipid bilayers - p. 273 19. Chapter Twelve: Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers - p. 293 20. Chapter Thirteen: The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture - p. 321
