Laboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. Laboratory Methods in Microbiology and Molecular Biology is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributor - p. iii 7. Authors' biographies - p. iii 8. Preface - p. iii 9. Acknowledgments - p. iii 10. Chapter 1. General guidelines, expectations, and responsibilities for inculcating good lab practices - p. 1 11. Chapter 2. Preparations of laboratory solutions - p. 7 12. Chapter 3. Basic concepts of microbiology - p. 21 13. Chapter 4. Microscopy and imaging analysis of microorganisms - p. 31 14. Chapter 5. Characterization of microbes using molecular methods - p. 45 15. Chapter 6. Soil microbiology - p. 59 16. Chapter 7. Basic experiments in food microbiology - p. 75 17. Chapter 8. Basic experiments in microbial biochemistry - p. 87 18. Chapter 9. Analytical techniques in microbial genomics - p. 105 19. Chapter 10. Analytical techniques in molecular biology-proteomics - p. 125 20. Chapter 11. Analytical techniques in proteomics - p. 135 21. Chapter 12. Expression of recombinant proteins in prokaryotic host - p. 145 22. Chapter 13. Sero-diagnostics in microbiology - p. 155 23. Chapter 14. Basic tools for next-generation DNA sequencing of bacterial genome - p. 167 24. Index - p. 177
Laboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. Laboratory Methods in Microbiology and Molecular Biology is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributor - p. iii 7. Authors' biographies - p. iii 8. Preface - p. iii 9. Acknowledgments - p. iii 10. Chapter 1. General guidelines, expectations, and responsibilities for inculcating good lab practices - p. 1 11. Chapter 2. Preparations of laboratory solutions - p. 7 12. Chapter 3. Basic concepts of microbiology - p. 21 13. Chapter 4. Microscopy and imaging analysis of microorganisms - p. 31 14. Chapter 5. Characterization of microbes using molecular methods - p. 45 15. Chapter 6. Soil microbiology - p. 59 16. Chapter 7. Basic experiments in food microbiology - p. 75 17. Chapter 8. Basic experiments in microbial biochemistry - p. 87 18. Chapter 9. Analytical techniques in microbial genomics - p. 105 19. Chapter 10. Analytical techniques in molecular biology-proteomics - p. 125 20. Chapter 11. Analytical techniques in proteomics - p. 135 21. Chapter 12. Expression of recombinant proteins in prokaryotic host - p. 145 22. Chapter 13. Sero-diagnostics in microbiology - p. 155 23. Chapter 14. Basic tools for next-generation DNA sequencing of bacterial genome - p. 167 24. Index - p. 177