Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science.
The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches.
Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research.
1. 1. Introduction to Microfluidics - p. 1 2. 2. Fabrication of a Glass Microfluidic Device - p. 9 3. 3. Fabrication of a Paper Microfluidic Device for Blood-Plasma Separation - p. 21 4. 4. Fabrication and Testing of a PDMS Microchip - p. 27 5. 5. Determination of Electroosmotic Flow in a Glass Microfluidic Device Using a Neutral Marker - p. 33 6. 6. Electrophoretic Separation in a Microchannel - p. 39 7. 7. A Simple Experiment for the Study of Droplet Microfluidics - p. 47 8. 8. Laminar Flow and Diffusion in a Microchannel - p. 51 9. 9. Beer’s Law Using a Smartphone and Paper Device - p. 57 10. 10. Acid–Base Titrations on Paper - p. 63 11. 11. Simultaneous Determination of Protein and Glucose in Urine Sample Using a Paper-Based Bioanalytical Device - p. 69 12. 12. Quantitative Determination of Total Amino Acids in Tea Using Paper Microfluidics and a Smartphone - p. 77 13. 13. Determination of Nitrite Ions in Water Using Paper Analytical Device - p. 83 14. 14. Colorimetric Determination of Multiple Metal Ions on µPAD - p. 89 15. 15. Analysis of a Mixture of Paracetamol and 4-Aminophenol in a Paper-Based Microfluidic Device - p. 97 16. 16. Synthesis of Gold Nanoparticles on Microchip - p. 103 17. 17. Flow Synthesis of Organic Dye on Microchip - p. 109 18. 18. Protein Immobilization on a Glass Microfluidic Channel - p. 115 19. 19. Microfluidic Enzyme-Linked Immunosorbent Assay - p. 121 20. Glossary - p. 129 21. Appendices - p. 131 22. Index - p. 155
Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science.
The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches.
Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research.
1. 1. Introduction to Microfluidics - p. 1 2. 2. Fabrication of a Glass Microfluidic Device - p. 9 3. 3. Fabrication of a Paper Microfluidic Device for Blood-Plasma Separation - p. 21 4. 4. Fabrication and Testing of a PDMS Microchip - p. 27 5. 5. Determination of Electroosmotic Flow in a Glass Microfluidic Device Using a Neutral Marker - p. 33 6. 6. Electrophoretic Separation in a Microchannel - p. 39 7. 7. A Simple Experiment for the Study of Droplet Microfluidics - p. 47 8. 8. Laminar Flow and Diffusion in a Microchannel - p. 51 9. 9. Beer’s Law Using a Smartphone and Paper Device - p. 57 10. 10. Acid–Base Titrations on Paper - p. 63 11. 11. Simultaneous Determination of Protein and Glucose in Urine Sample Using a Paper-Based Bioanalytical Device - p. 69 12. 12. Quantitative Determination of Total Amino Acids in Tea Using Paper Microfluidics and a Smartphone - p. 77 13. 13. Determination of Nitrite Ions in Water Using Paper Analytical Device - p. 83 14. 14. Colorimetric Determination of Multiple Metal Ions on µPAD - p. 89 15. 15. Analysis of a Mixture of Paracetamol and 4-Aminophenol in a Paper-Based Microfluidic Device - p. 97 16. 16. Synthesis of Gold Nanoparticles on Microchip - p. 103 17. 17. Flow Synthesis of Organic Dye on Microchip - p. 109 18. 18. Protein Immobilization on a Glass Microfluidic Channel - p. 115 19. 19. Microfluidic Enzyme-Linked Immunosorbent Assay - p. 121 20. Glossary - p. 129 21. Appendices - p. 131 22. Index - p. 155
