Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play a key role in the oxidation of phenolic substrates and an important biological function in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few practical resources exist on their fundamental biology, research methods, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering a practical overview of these important enzyme classes, and empowering researchers to develop their own studies and hone applications. The book deftly connects topics across these intricate enzyme systems, with early chapters on physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes’ function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to healthcare, bioremediation, and green and computational chemistry. Step-by-step instructions and explanation offer clear guidance in replicating key research and application methodologies discussed.
1. Laccase and Polyphenol Oxidase - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Foundations and frontiers in enzymology series - p. Page iv 6. Copyright - p. Page v 7. Contributors - p. Page v 8. About the editor - p. Page viii 9. Preface - p. Page ix 10. List of Illustrations - p. Page x 11. List of Tables - p. Page xi 12. Chapter 1 : Functional roles of polyphenol oxidases in plants and fungi - p. Page xi 13. Chapter 2 : Plant PPO: Production, purification, and activity assays - p. Page 26 14. Chapter 3 : Laccase and polyphenol oxidase: Biochemistry and biotechnological applications - p. Page 64 15. Chapter 4 : Recent advances in the design of PPO inhibitors - p. Page 79 16. Chapter 5 : Reaction mechanism and redox potential of laccase - p. Page 103 17. Chapter 6 : Laccases: Biological functions and potential applications - p. Page 126 18. Chapter 7 : Challenges and applications of laccase in bioremediation - p. Page 143 19. Chapter 8 : Immobilization of laccase for industrial wastewater treatment: Current challenges and future perspectives - p. Page 173 20. Chapter 9 : In silico bioprospecting and engineering laccases: Methods and applications - p. Page 229 21. Author Index - p. Page 263 22. Subject Index - p. Page 280
Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play a key role in the oxidation of phenolic substrates and an important biological function in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few practical resources exist on their fundamental biology, research methods, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering a practical overview of these important enzyme classes, and empowering researchers to develop their own studies and hone applications. The book deftly connects topics across these intricate enzyme systems, with early chapters on physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes’ function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to healthcare, bioremediation, and green and computational chemistry. Step-by-step instructions and explanation offer clear guidance in replicating key research and application methodologies discussed.
1. Laccase and Polyphenol Oxidase - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Foundations and frontiers in enzymology series - p. Page iv 6. Copyright - p. Page v 7. Contributors - p. Page v 8. About the editor - p. Page viii 9. Preface - p. Page ix 10. List of Illustrations - p. Page x 11. List of Tables - p. Page xi 12. Chapter 1 : Functional roles of polyphenol oxidases in plants and fungi - p. Page xi 13. Chapter 2 : Plant PPO: Production, purification, and activity assays - p. Page 26 14. Chapter 3 : Laccase and polyphenol oxidase: Biochemistry and biotechnological applications - p. Page 64 15. Chapter 4 : Recent advances in the design of PPO inhibitors - p. Page 79 16. Chapter 5 : Reaction mechanism and redox potential of laccase - p. Page 103 17. Chapter 6 : Laccases: Biological functions and potential applications - p. Page 126 18. Chapter 7 : Challenges and applications of laccase in bioremediation - p. Page 143 19. Chapter 8 : Immobilization of laccase for industrial wastewater treatment: Current challenges and future perspectives - p. Page 173 20. Chapter 9 : In silico bioprospecting and engineering laccases: Methods and applications - p. Page 229 21. Author Index - p. Page 263 22. Subject Index - p. Page 280
