Lipids and Membranes: Detection by Imaging and Lipidomics, Volume 727 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the chapters in this new release include Measurement of fatty acid esters of hydroxy fatty acids, Superresolution organelle imaging with lipophilic HIDE probes, Ultra-high resolution analysis of membrane lipids from 15N metabolically labeled mice, Superresolution lipid imaging with expansion microscopy, High-resolution phosphoinositide analysis in healthy versus disease cells, and much more.
Additional chapters focus on Alkyne lipids as tracers of lipid metabolism, Imaging oxidative stress in biomembranes, Imaging the dynamics of phosphatidylinositol 4-phosphate metabolism, Lipidomics for studying sphingolipids in cell death, Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors, Profiling lipotypes at the single cell level in heterogenous populations, MS imaging of phosphoinositides, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: Expanding the multiplexing capability of HIDE probes via fluorescence lifetime imaging microscopy - p. 1 9. Chapter Two: Expansion microscopy (ExM) of lipids for super-resolution imaging of membranes - p. 19 10. Chapter Three: High-resolution phosphoinositide analysis - p. 45 11. Chapter Four: Alkyne lipids as tracers of lipid metabolism - p. 85 12. Chapter Five: Imaging oxidative and nitrative stress in membranes - p. 105 13. Chapter Six: Spatiotemporal analysis of phosphatidylinositol 4-phosphate dynamics - p. 135 14. Chapter Seven: High-resolution sphingolipid analysis in vitro using LC-qToF MS - p. 149 15. Chapter Eight: Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors - p. 173 16. Chapter Nine: Visualization of single-cell lipidomes with MALDI-MSI - p. 185 17. Chapter Ten: Detection, imaging and quantification of phosphoinositides using ion chromatography suppressed conductivity and mass spectrometry imaging - p. 209 18. Chapter Eleven: Strategies to minimize artificial lipid oxidation in mass spectrometry based epilipidomics analysis - p. 245 19. Chapter Twelve: Quantitative analysis of H-Ras localization using confocal microscopy - p. 261 20. Chapter Thirteen: Metabolic labeling of glycerophospholipids - p. 281 21. Chapter Fourteen: Characterization of human Akt1-lipid interactions by label-free differential scanning fluorimetry - p. 313 22. Chapter Fifteen: Mass spectrometry-based lipid analysis in NPC1 disease: Methods for phosphoinositide quantification, lipid imaging, and myelin lipid profiling - p. 325 23. Chapter Sixteen: Multi-structural profiling of neutral glycosphingolipids using selective enrichment and the Paternò–Büchi derivatization - p. 349
Lipids and Membranes: Detection by Imaging and Lipidomics, Volume 727 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the chapters in this new release include Measurement of fatty acid esters of hydroxy fatty acids, Superresolution organelle imaging with lipophilic HIDE probes, Ultra-high resolution analysis of membrane lipids from 15N metabolically labeled mice, Superresolution lipid imaging with expansion microscopy, High-resolution phosphoinositide analysis in healthy versus disease cells, and much more.
Additional chapters focus on Alkyne lipids as tracers of lipid metabolism, Imaging oxidative stress in biomembranes, Imaging the dynamics of phosphatidylinositol 4-phosphate metabolism, Lipidomics for studying sphingolipids in cell death, Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors, Profiling lipotypes at the single cell level in heterogenous populations, MS imaging of phosphoinositides, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: Expanding the multiplexing capability of HIDE probes via fluorescence lifetime imaging microscopy - p. 1 9. Chapter Two: Expansion microscopy (ExM) of lipids for super-resolution imaging of membranes - p. 19 10. Chapter Three: High-resolution phosphoinositide analysis - p. 45 11. Chapter Four: Alkyne lipids as tracers of lipid metabolism - p. 85 12. Chapter Five: Imaging oxidative and nitrative stress in membranes - p. 105 13. Chapter Six: Spatiotemporal analysis of phosphatidylinositol 4-phosphate dynamics - p. 135 14. Chapter Seven: High-resolution sphingolipid analysis in vitro using LC-qToF MS - p. 149 15. Chapter Eight: Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors - p. 173 16. Chapter Nine: Visualization of single-cell lipidomes with MALDI-MSI - p. 185 17. Chapter Ten: Detection, imaging and quantification of phosphoinositides using ion chromatography suppressed conductivity and mass spectrometry imaging - p. 209 18. Chapter Eleven: Strategies to minimize artificial lipid oxidation in mass spectrometry based epilipidomics analysis - p. 245 19. Chapter Twelve: Quantitative analysis of H-Ras localization using confocal microscopy - p. 261 20. Chapter Thirteen: Metabolic labeling of glycerophospholipids - p. 281 21. Chapter Fourteen: Characterization of human Akt1-lipid interactions by label-free differential scanning fluorimetry - p. 313 22. Chapter Fifteen: Mass spectrometry-based lipid analysis in NPC1 disease: Methods for phosphoinositide quantification, lipid imaging, and myelin lipid profiling - p. 325 23. Chapter Sixteen: Multi-structural profiling of neutral glycosphingolipids using selective enrichment and the Paternò–Büchi derivatization - p. 349
