Lipids and Membranes: Dynamics and Interorganelle Lipid Transport, Volume 728 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the book's chapters include topics such as Assay of phospholipid scrambling, Tracking lipid transport with bifunctional lipids, Tracking interorganelle lipid transport using genetically encoded mass tags, Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains, Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation, Studying interactions of amphipathic helices with lipid droplets, and more.
Additional chapters cover Metabolic labeling of to trace changes in sphingolipid metabolism, Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing, Tagging lipoproteins in mycobacteria, Probes for studying sphingolipid dynamics, Measuring Flipper-TR Lifetime with Multiple Analysis Strategies, Reconstitution approaches to understanding cellular lipid dynamics, Prediction and characterization of the bacterial lipid handling machinery, From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: Regulation of phospholipid scramblases by cholesterol - p. 1 9. Chapter Two: Lipid imaging using bifunctional lipid probes - p. 17 10. Chapter Three: Docking and molecular dynamics simulations of ORPphilins targeting OSBP - p. 47 11. Chapter Four: Imaging and quantifying organelle contact sites using a reversible splitFAST complementation system - p. 75 12. Chapter Five: In vitro methods for studying protein-lipid droplet interactions - p. 93 13. Chapter Six: Tracing sphingolipid metabolism kinetics using radioactive and non-radioactive labels - p. 123 14. Chapter Seven: Imaging intracellular lipid heterogeneity with fluorogen-activating coincidence encounter sensing - p. 145 15. Chapter Eight: Tagging lipoproteins in mycobacteria via metabolic incorporation of modified fatty acids - p. 161 16. Chapter Nine: Fluorescence lifetime estimation: A practical approach using Flipper-TR FLIM - p. 179 17. Chapter Ten: Reconstitution approaches for understanding cellular lipid dynamics - p. 233 18. Chapter Eleven: Prediction and characterization of lipid-interacting proteins - p. 253 19. Chapter Twelve: From molecular dynamics to cryo-EM: Imaging liposomes in silico - p. 291 20. Chapter Thirteen: Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins - p. 321 21. Chapter Fourteen: Organelle-selective click chemistry for monitoring fatty acid metabolism at the subcellular level - p. 355 22. Chapter Fifteen: In vitro methods for measuring lipid binding, transfer and competitive displacement reactions by peripheral membrane proteins - p. 373 23. Chapter Sixteen: Purification of the lipid transfer protein tricalbin 3 in a native environment by using an amphiphilic copolymer - p. 417 24. Chapter Seventeen: Use of small unilamellar vesicles to study the biochemical properties of the GRAM domain of GRAMD1 - p. 429
Lipids and Membranes: Dynamics and Interorganelle Lipid Transport, Volume 728 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the book's chapters include topics such as Assay of phospholipid scrambling, Tracking lipid transport with bifunctional lipids, Tracking interorganelle lipid transport using genetically encoded mass tags, Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains, Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation, Studying interactions of amphipathic helices with lipid droplets, and more.
Additional chapters cover Metabolic labeling of to trace changes in sphingolipid metabolism, Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing, Tagging lipoproteins in mycobacteria, Probes for studying sphingolipid dynamics, Measuring Flipper-TR Lifetime with Multiple Analysis Strategies, Reconstitution approaches to understanding cellular lipid dynamics, Prediction and characterization of the bacterial lipid handling machinery, From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: Regulation of phospholipid scramblases by cholesterol - p. 1 9. Chapter Two: Lipid imaging using bifunctional lipid probes - p. 17 10. Chapter Three: Docking and molecular dynamics simulations of ORPphilins targeting OSBP - p. 47 11. Chapter Four: Imaging and quantifying organelle contact sites using a reversible splitFAST complementation system - p. 75 12. Chapter Five: In vitro methods for studying protein-lipid droplet interactions - p. 93 13. Chapter Six: Tracing sphingolipid metabolism kinetics using radioactive and non-radioactive labels - p. 123 14. Chapter Seven: Imaging intracellular lipid heterogeneity with fluorogen-activating coincidence encounter sensing - p. 145 15. Chapter Eight: Tagging lipoproteins in mycobacteria via metabolic incorporation of modified fatty acids - p. 161 16. Chapter Nine: Fluorescence lifetime estimation: A practical approach using Flipper-TR FLIM - p. 179 17. Chapter Ten: Reconstitution approaches for understanding cellular lipid dynamics - p. 233 18. Chapter Eleven: Prediction and characterization of lipid-interacting proteins - p. 253 19. Chapter Twelve: From molecular dynamics to cryo-EM: Imaging liposomes in silico - p. 291 20. Chapter Thirteen: Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins - p. 321 21. Chapter Fourteen: Organelle-selective click chemistry for monitoring fatty acid metabolism at the subcellular level - p. 355 22. Chapter Fifteen: In vitro methods for measuring lipid binding, transfer and competitive displacement reactions by peripheral membrane proteins - p. 373 23. Chapter Sixteen: Purification of the lipid transfer protein tricalbin 3 in a native environment by using an amphiphilic copolymer - p. 417 24. Chapter Seventeen: Use of small unilamellar vesicles to study the biochemical properties of the GRAM domain of GRAMD1 - p. 429
