Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter One: Using a sequence-specific coarse-grained model for studying protein liquid–liquid phase separation - p. page_1 8. Chapter Two: Reconstituting bacterial cell division assemblies in crowded, phase-separated media - p. page_19 9. Chapter Three: Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform - p. page_51 10. Chapter Four: Cell-free reconstitution of multi-condensate assemblies - p. page_83 11. Chapter Five: Practical considerations for generation of multi-compartment complex coacervates - p. page_115 12. Chapter Six: Methods for characterizing the material properties of biomolecular condensates - p. page_143 13. Chapter Seven: Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit - p. page_185 14. Chapter Eight: SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes - p. page_223 15. Chapter Nine: Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates - p. page_261 16. Chapter Ten: Incorporation of proteins into complex coacervates - p. page_277 17. Chapter Eleven: Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation - p. page_307 18. Chapter Twelve: Reversible photocontrol of DNA coacervation - p. page_329 19. Chapter Thirteen: Enzymatic control over coacervation - p. page_353
Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter One: Using a sequence-specific coarse-grained model for studying protein liquid–liquid phase separation - p. page_1 8. Chapter Two: Reconstituting bacterial cell division assemblies in crowded, phase-separated media - p. page_19 9. Chapter Three: Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform - p. page_51 10. Chapter Four: Cell-free reconstitution of multi-condensate assemblies - p. page_83 11. Chapter Five: Practical considerations for generation of multi-compartment complex coacervates - p. page_115 12. Chapter Six: Methods for characterizing the material properties of biomolecular condensates - p. page_143 13. Chapter Seven: Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit - p. page_185 14. Chapter Eight: SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes - p. page_223 15. Chapter Nine: Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates - p. page_261 16. Chapter Ten: Incorporation of proteins into complex coacervates - p. page_277 17. Chapter Eleven: Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation - p. page_307 18. Chapter Twelve: Reversible photocontrol of DNA coacervation - p. page_329 19. Chapter Thirteen: Enzymatic control over coacervation - p. page_353
