Marine enzymes and specialized metabolism - Part A, Volume 604 highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. These detailed protocols are written by experts who actively study and apply marine enzymes in biosynthesis and biotechnology. Comprehensive chapters in this latest release cover Chemoenzymatic synthesis of starting materials and characterization of halogenases requiring acyl carrier protein tethered substrates, Assaying biradical aryl coupling activity of CYP450 enzymes, the Characterization and application of marine microbial omega-3 polyunsaturated fatty acid synthesis, Catalase-related allene oxide synthase: on a biosynthetic route to fatty acid cyclopentenones, Haloalkane dehalogenases from marine microorganisms, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Marine Cyanobacteria, Enzymes and Pathways - p. xvi 8. Chapter One: Collection, Culturing, and Genome Analyses of Tropical Marine Filamentous Benthic Cyanobacteria - p. xvi 9. Chapter Two: PKS–NRPS Enzymology and Structural Biology: Considerations in Protein Production - p. 43 10. Chapter Three: Terminal Alkyne Biosynthesis in Marine Microbes - p. 88 11. Chapter Four: The Biochemistry and Structural Biology of Cyanobactin Pathways: Enabling Combinatorial Biosynthesis - p. 112 12. Chapter Five: The Enzymology of Prochlorosin Biosynthesis - p. 163 13. Section II: Biosynthesis by Bacterial Symbionts of Marine Invertebrate Animals - p. 203 14. Chapter Six: Chemoenzymatic Dissection of Polyketide β-Branching in the Bryostatin Pathway - p. 203 15. Chapter Seven: Radical S-Adenosylmethionine Peptide Epimerases: Detection of Activity and Characterization of d-Amino Acid Products - p. 236 16. Chapter Eight: Cobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression - p. 257 17. Chapter Nine: Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in “Candidatus Entotheonella sp.” - p. 286 18. Section III: Natural Product Biosynthetic Enzymology of Marine Bacteria and Fungi - p. 330 19. Chapter Ten: Chemoenzymatic Synthesis of Starting Materials and Characterization of Halogenases Requiring Acyl Carrier Protein-Tethered Substrates - p. 330 20. Chapter Eleven: Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-l-Methionine and Analogs - p. 366 21. Chapter Twelve: In Vitro Analysis of Cyanobacterial Nonheme Iron-Dependent Aliphatic Halogenases WelO5 and AmbO5 - p. 388 22. Chapter Thirteen: Characterization and Biochemical Assays of Streptomyces Vanadium-Dependent Chloroperoxidases - p. 404 23. Chapter Fourteen: Unusual “Head-to-Torso” Coupling of Terpene Precursors as a New Strategy for the Structural Diversification of Natural Products - p. 424 24. Chapter Fifteen: Identification of Enzymes Involved in Sesterterpene Biosynthesis in Marine Fungi - p. 439 25. Chapter Sixteen: Assaying Oxidative Coupling Activity of CYP450 Enzymes - p. 498 26. Chapter Seventeen: Preparation and Characterization of the Favorskiiase Flavoprotein EncM and Its Distinctive Flavin-N5-Oxide Cofactor - p. 521
Marine enzymes and specialized metabolism - Part A, Volume 604 highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. These detailed protocols are written by experts who actively study and apply marine enzymes in biosynthesis and biotechnology. Comprehensive chapters in this latest release cover Chemoenzymatic synthesis of starting materials and characterization of halogenases requiring acyl carrier protein tethered substrates, Assaying biradical aryl coupling activity of CYP450 enzymes, the Characterization and application of marine microbial omega-3 polyunsaturated fatty acid synthesis, Catalase-related allene oxide synthase: on a biosynthetic route to fatty acid cyclopentenones, Haloalkane dehalogenases from marine microorganisms, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Marine Cyanobacteria, Enzymes and Pathways - p. xvi 8. Chapter One: Collection, Culturing, and Genome Analyses of Tropical Marine Filamentous Benthic Cyanobacteria - p. xvi 9. Chapter Two: PKS–NRPS Enzymology and Structural Biology: Considerations in Protein Production - p. 43 10. Chapter Three: Terminal Alkyne Biosynthesis in Marine Microbes - p. 88 11. Chapter Four: The Biochemistry and Structural Biology of Cyanobactin Pathways: Enabling Combinatorial Biosynthesis - p. 112 12. Chapter Five: The Enzymology of Prochlorosin Biosynthesis - p. 163 13. Section II: Biosynthesis by Bacterial Symbionts of Marine Invertebrate Animals - p. 203 14. Chapter Six: Chemoenzymatic Dissection of Polyketide β-Branching in the Bryostatin Pathway - p. 203 15. Chapter Seven: Radical S-Adenosylmethionine Peptide Epimerases: Detection of Activity and Characterization of d-Amino Acid Products - p. 236 16. Chapter Eight: Cobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression - p. 257 17. Chapter Nine: Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in “Candidatus Entotheonella sp.” - p. 286 18. Section III: Natural Product Biosynthetic Enzymology of Marine Bacteria and Fungi - p. 330 19. Chapter Ten: Chemoenzymatic Synthesis of Starting Materials and Characterization of Halogenases Requiring Acyl Carrier Protein-Tethered Substrates - p. 330 20. Chapter Eleven: Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-l-Methionine and Analogs - p. 366 21. Chapter Twelve: In Vitro Analysis of Cyanobacterial Nonheme Iron-Dependent Aliphatic Halogenases WelO5 and AmbO5 - p. 388 22. Chapter Thirteen: Characterization and Biochemical Assays of Streptomyces Vanadium-Dependent Chloroperoxidases - p. 404 23. Chapter Fourteen: Unusual “Head-to-Torso” Coupling of Terpene Precursors as a New Strategy for the Structural Diversification of Natural Products - p. 424 24. Chapter Fifteen: Identification of Enzymes Involved in Sesterterpene Biosynthesis in Marine Fungi - p. 439 25. Chapter Sixteen: Assaying Oxidative Coupling Activity of CYP450 Enzymes - p. 498 26. Chapter Seventeen: Preparation and Characterization of the Favorskiiase Flavoprotein EncM and Its Distinctive Flavin-N5-Oxide Cofactor - p. 521
