Metagenomics: Perspectives, Methods, and Applications provides thorough coverage of the growing field of metagenomics. A diverse range of chapters from international experts offer an introduction to the field and examine methods for metagenomic analysis of microbiota, metagenomic computational tools, and recent metagenomic studies in various environments. The emphasis on application makes this text particularly useful for applied researchers, practitioners, clinicians and students seeking to employ metagenomic approaches to advance knowledge in the biomedical and life sciences. Case-study based application chapters examine topics ranging from viral metagenome profiling, metagenomics in oral disease and health, metagenomic insights into the human gut microbiome and metabolic syndromes, and more.
Additionally, perspectives on future potential at the end of each chapter provoke new thought and motivations for continued study in this exciting and fruitful research area.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Foreword - p. xvii 7. Preface - p. xix 8. Acknowledgments - p. xxi 9. Chapter 1: Metagenomics: A Paradigm Shift in Microbiology - p. xxi 10. Chapter 2: Metagenomic Protocols and Strategies - p. 13 11. Chapter 3: Strategies for Taxonomic and Functional Annotation of Metagenomes - p. 54 12. Chapter 4: Computational and Statistical Considerations in the Analysis of Metagenomic Data - p. 79 13. Chapter 5: 16S rRNA-Based Taxonomy Profiling in the Metagenomics Era - p. 102 14. Chapter 6: Analyzing High-Throughput Microbial Amplicon Sequence Data Using Multiple Markers - p. 119 15. Chapter 7: Human Body to Water Reservoirs: Estimating Viral Population Characteristics Using High-Throughput Sequencing - p. 138 16. Chapter 8: Measuring Microbiome Diversity and Similarity with Hill Numbers - p. 155 17. Chapter 9: Metagenomics: Implications in Oral Health and Disease - p. 178 18. Chapter 10: The Human Gut Microbiome in Health and Disease - p. 195 19. Chapter 11: Comprehensive Exploration of the Rumen Microbial Ecosystem With Advancements in Metagenomics - p. 213 20. Chapter 12: The Microbiome of Desert CAM Plants: Lessons From Amplicon Sequencing and Metagenomics - p. 229 21. Chapter 13: Metagenomics of Marine Invertebrate-Microbial Consortium - p. 254 22. Chapter 14: Novel Biological Resources Screened From Uncultured Bacteria by a Metagenomic Method - p. 272 23. Chapter 15: Advances of Functional Metagenomics in Harnessing Thermozymes - p. 288 24. Chapter 16: Direct Cellulase Gene Amplification From Hot Spring Using the Guidance of 16S rRNA Amplicon Metagenomics - p. 307 25. Chapter 17: Comparative Metaproteomics to Study Environmental Changes - p. 325 26. Index - p. 365
Metagenomics: Perspectives, Methods, and Applications provides thorough coverage of the growing field of metagenomics. A diverse range of chapters from international experts offer an introduction to the field and examine methods for metagenomic analysis of microbiota, metagenomic computational tools, and recent metagenomic studies in various environments. The emphasis on application makes this text particularly useful for applied researchers, practitioners, clinicians and students seeking to employ metagenomic approaches to advance knowledge in the biomedical and life sciences. Case-study based application chapters examine topics ranging from viral metagenome profiling, metagenomics in oral disease and health, metagenomic insights into the human gut microbiome and metabolic syndromes, and more.
Additionally, perspectives on future potential at the end of each chapter provoke new thought and motivations for continued study in this exciting and fruitful research area.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Foreword - p. xvii 7. Preface - p. xix 8. Acknowledgments - p. xxi 9. Chapter 1: Metagenomics: A Paradigm Shift in Microbiology - p. xxi 10. Chapter 2: Metagenomic Protocols and Strategies - p. 13 11. Chapter 3: Strategies for Taxonomic and Functional Annotation of Metagenomes - p. 54 12. Chapter 4: Computational and Statistical Considerations in the Analysis of Metagenomic Data - p. 79 13. Chapter 5: 16S rRNA-Based Taxonomy Profiling in the Metagenomics Era - p. 102 14. Chapter 6: Analyzing High-Throughput Microbial Amplicon Sequence Data Using Multiple Markers - p. 119 15. Chapter 7: Human Body to Water Reservoirs: Estimating Viral Population Characteristics Using High-Throughput Sequencing - p. 138 16. Chapter 8: Measuring Microbiome Diversity and Similarity with Hill Numbers - p. 155 17. Chapter 9: Metagenomics: Implications in Oral Health and Disease - p. 178 18. Chapter 10: The Human Gut Microbiome in Health and Disease - p. 195 19. Chapter 11: Comprehensive Exploration of the Rumen Microbial Ecosystem With Advancements in Metagenomics - p. 213 20. Chapter 12: The Microbiome of Desert CAM Plants: Lessons From Amplicon Sequencing and Metagenomics - p. 229 21. Chapter 13: Metagenomics of Marine Invertebrate-Microbial Consortium - p. 254 22. Chapter 14: Novel Biological Resources Screened From Uncultured Bacteria by a Metagenomic Method - p. 272 23. Chapter 15: Advances of Functional Metagenomics in Harnessing Thermozymes - p. 288 24. Chapter 16: Direct Cellulase Gene Amplification From Hot Spring Using the Guidance of 16S rRNA Amplicon Metagenomics - p. 307 25. Chapter 17: Comparative Metaproteomics to Study Environmental Changes - p. 325 26. Index - p. 365
