Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis.
This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter 1: Ultrastructural analysis of the extracellular matrix - p. xxi 8. Chapter 2: Live tissue antibody injection: A novel method for imaging ECM in limb buds and other tissues - p. 39 9. Chapter 3: Nonlinear optical imaging of extracellular matrix proteins - p. 56 10. Chapter 4: Methods for the visualization and analysis of extracellular matrix protein structure and degradation - p. 78 11. Chapter 5: Cell-derived decellularized extracellular matrices - p. 95 12. Chapter 6: Analysis of collagen and elastin cross-links - p. 114 13. Chapter 7: Measurement of elastin, collagen, and total protein levels in tissues - p. 132 14. Chapter 8: Measurement of lysyl oxidase activity from small tissue samples and cell cultures - p. 146 15. Chapter 9: Microtiter assays for quantitation of assembly of plasma and cellular fibronectin - p. 156 16. Chapter 10: Basement membrane collagen IV: Isolation of functional domains - p. 170 17. Chapter 11: Isolation and analysis of laminins - p. 185 18. Chapter 12: Elastin purification and solubilization - p. 205 19. Chapter 13: Fibrillins - p. 222 20. Chapter 14: Expression and purification of recombinant fibulins in mammalian cells - p. 246 21. Chapter 15: The biochemistry and immunohistochemistry of versican - p. 259 22. Chapter 16: Isolation, production, and analysis of small leucine-rich proteoglycans in bone - p. 279 23. Chapter 17: Quantification of hyaluronan (HA) using a simplified fluorophore-assisted carbohydrate electrophoresis (FACE) procedure☆ - p. 296 24. Chapter 18: Isolation and functional analysis of syndecans - p. 316 25. Chapter 19: Production and purification of recombinant human SPARC - p. 333 26. Chapter 20: Thrombospondins: Purification of human platelet thrombospondin-1 - p. 345 27. Chapter 21: How to detect and purify tenascin-C - p. 369 28. Chapter 22: Investigating cell-type specific functions of tenascin-C - p. 400 29. Chapter 23: Matrilins - p. 428
Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis.
This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter 1: Ultrastructural analysis of the extracellular matrix - p. xxi 8. Chapter 2: Live tissue antibody injection: A novel method for imaging ECM in limb buds and other tissues - p. 39 9. Chapter 3: Nonlinear optical imaging of extracellular matrix proteins - p. 56 10. Chapter 4: Methods for the visualization and analysis of extracellular matrix protein structure and degradation - p. 78 11. Chapter 5: Cell-derived decellularized extracellular matrices - p. 95 12. Chapter 6: Analysis of collagen and elastin cross-links - p. 114 13. Chapter 7: Measurement of elastin, collagen, and total protein levels in tissues - p. 132 14. Chapter 8: Measurement of lysyl oxidase activity from small tissue samples and cell cultures - p. 146 15. Chapter 9: Microtiter assays for quantitation of assembly of plasma and cellular fibronectin - p. 156 16. Chapter 10: Basement membrane collagen IV: Isolation of functional domains - p. 170 17. Chapter 11: Isolation and analysis of laminins - p. 185 18. Chapter 12: Elastin purification and solubilization - p. 205 19. Chapter 13: Fibrillins - p. 222 20. Chapter 14: Expression and purification of recombinant fibulins in mammalian cells - p. 246 21. Chapter 15: The biochemistry and immunohistochemistry of versican - p. 259 22. Chapter 16: Isolation, production, and analysis of small leucine-rich proteoglycans in bone - p. 279 23. Chapter 17: Quantification of hyaluronan (HA) using a simplified fluorophore-assisted carbohydrate electrophoresis (FACE) procedure☆ - p. 296 24. Chapter 18: Isolation and functional analysis of syndecans - p. 316 25. Chapter 19: Production and purification of recombinant human SPARC - p. 333 26. Chapter 20: Thrombospondins: Purification of human platelet thrombospondin-1 - p. 345 27. Chapter 21: How to detect and purify tenascin-C - p. 369 28. Chapter 22: Investigating cell-type specific functions of tenascin-C - p. 400 29. Chapter 23: Matrilins - p. 428
