Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter 1: Orthotopic brain tumor models derived from glioblastoma stem-like cells - p. 1 7. Chapter 2: Identification and isolation of slow-cycling glioma stem cells - p. 21 8. Chapter 3: Immunohistochemical analysis of the developing mouse cortex - p. 31 9. Chapter 4: Subcellular fractionation of brain tumor stem cells - p. 47 10. Chapter 5: microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting - p. 59 11. Chapter 6: Genome-wide RNA interference screen in cancer stem cells - p. 81 12. Chapter 7: Clonal analysis of human clonogenic keratinocytes - p. 101 13. Chapter 8: Immunofluorescence analysis of myogenic differentiation - p. 117 14. Chapter 9: Generating pancreatic beta-like cells from human pluripotent stem cells - p. 127 15. Chapter 10: In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering - p. 147 16. Chapter 11: Identification of progenitor cells and their progenies in adult Drosophila midgut - p. 169 17. Chapter 12: Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications - p. 189 18. Chapter 13: Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells - p. 203
Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter 1: Orthotopic brain tumor models derived from glioblastoma stem-like cells - p. 1 7. Chapter 2: Identification and isolation of slow-cycling glioma stem cells - p. 21 8. Chapter 3: Immunohistochemical analysis of the developing mouse cortex - p. 31 9. Chapter 4: Subcellular fractionation of brain tumor stem cells - p. 47 10. Chapter 5: microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting - p. 59 11. Chapter 6: Genome-wide RNA interference screen in cancer stem cells - p. 81 12. Chapter 7: Clonal analysis of human clonogenic keratinocytes - p. 101 13. Chapter 8: Immunofluorescence analysis of myogenic differentiation - p. 117 14. Chapter 9: Generating pancreatic beta-like cells from human pluripotent stem cells - p. 127 15. Chapter 10: In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering - p. 147 16. Chapter 11: Identification of progenitor cells and their progenies in adult Drosophila midgut - p. 169 17. Chapter 12: Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications - p. 189 18. Chapter 13: Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells - p. 203
