Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Section I: Tau Structure, Oligomerization and Aggregation in Vitro - p. xix 8. Chapter 1: Expression and isolation of recombinant tau - p. xix 9. Chapter 2: Insights into tau function and dysfunction through single-molecule fluorescence - p. 26 10. Chapter 3: Production of recombinant tau oligomers in vitro - p. 44 11. Chapter 4: Optimization of in vitro conditions to study the arachidonic acid induction of 4R isoforms of the microtubule-associated protein tau - p. 64 12. Chapter 5: Conformation-based assay of tau protein aggregation - p. 88 13. Section II: Tau-Microtubule Interactions - p. 111 14. Chapter 6: Measuring Tau–microtubule affinity through cosedimentation assays - p. 111 15. Chapter 7: Single-molecule imaging of Tau dynamics on the microtubule surface - p. 134 16. Chapter 8: Synchrotron small-angle X-ray scattering and electron microscopy characterization of structures and forces in microtubule/Tau mixtures - p. 154 17. Chapter 9: A TIRF microscopy assay to decode how tau regulates EB’s tracking at microtubule ends - p. 178 18. Chapter 10: TIRF assays for real-time observation of microtubules and actin coassembly: Deciphering tau effects on microtubule/actin interplay - p. 197 19. Section III: Cells and Model Systems - p. 214 20. Chapter 11: Digital quantification of neurite outgrowth and retraction by phase-contrast microscopy: A tau perspective - p. 214 21. Chapter 12: Assessing the degradation of tau in primary neurons: The role of autophagy - p. 228 22. Chapter 13: Methods related to studying tau fragmentation - p. 244 23. Chapter 14: The Drosophila model system to study tau action - p. 258 24. Chapter 15: The use of mouse models to study cell-to-cell transmission of pathological tau - p. 286 25. Chapter 16: Studying tau protein propagation and pathology in the mouse brain using adeno-associated viruses - p. 305
Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Section I: Tau Structure, Oligomerization and Aggregation in Vitro - p. xix 8. Chapter 1: Expression and isolation of recombinant tau - p. xix 9. Chapter 2: Insights into tau function and dysfunction through single-molecule fluorescence - p. 26 10. Chapter 3: Production of recombinant tau oligomers in vitro - p. 44 11. Chapter 4: Optimization of in vitro conditions to study the arachidonic acid induction of 4R isoforms of the microtubule-associated protein tau - p. 64 12. Chapter 5: Conformation-based assay of tau protein aggregation - p. 88 13. Section II: Tau-Microtubule Interactions - p. 111 14. Chapter 6: Measuring Tau–microtubule affinity through cosedimentation assays - p. 111 15. Chapter 7: Single-molecule imaging of Tau dynamics on the microtubule surface - p. 134 16. Chapter 8: Synchrotron small-angle X-ray scattering and electron microscopy characterization of structures and forces in microtubule/Tau mixtures - p. 154 17. Chapter 9: A TIRF microscopy assay to decode how tau regulates EB’s tracking at microtubule ends - p. 178 18. Chapter 10: TIRF assays for real-time observation of microtubules and actin coassembly: Deciphering tau effects on microtubule/actin interplay - p. 197 19. Section III: Cells and Model Systems - p. 214 20. Chapter 11: Digital quantification of neurite outgrowth and retraction by phase-contrast microscopy: A tau perspective - p. 214 21. Chapter 12: Assessing the degradation of tau in primary neurons: The role of autophagy - p. 228 22. Chapter 13: Methods related to studying tau fragmentation - p. 244 23. Chapter 14: The Drosophila model system to study tau action - p. 258 24. Chapter 15: The use of mouse models to study cell-to-cell transmission of pathological tau - p. 286 25. Chapter 16: Studying tau protein propagation and pathology in the mouse brain using adeno-associated viruses - p. 305
