MicroRNA: From Bench to Bedside provides an in-depth, expansive overview of microRNA from fundamentals to clinical practice. It presents researchers with detailed insights on the topic of microRNA, exploring foundational knowledge that is followed by methodologies and the latest technologies for research and potential theragnostic and therapeutic applications for specific diseases. The book consists of eight parts, beginning with an introduction to microRNA and the current state of the field, followed by sections on biogenesis and maturation of microRNA, and methodology and bioinformatics, where chapters focus on isolation and detection techniques.
Sections then move on to molecular mechanisms and gene regulation, considering topics such as transcriptional regulation and epigenetic regulation, as well as the role of microRNA as biomarkers. Additionally, microRNA and human disease and microRNA-based therapeutics are explored, focusing on a wide range of diseases such as cancer, age-related disease, cardiovascular disease, microRNA targeted therapy in hepatitis and therapeutic strategies for diabetes. The book concludes with a discussion on advances and future perspectives in microRNA investigation.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. xiii 7. Preface - p. xix 8. Part I: Overview - p. xx 9. Chapter 1. Overview of micro-RNA - p. 3 10. Part II: Biogenesis and maturation - p. 11 11. Chapter 2. Maturation of microRNAs - p. 19 12. Part III: Methodology and bioinformatics - p. 27 13. Chapter 3. MicroRNA interference - p. 33 14. Chapter 4. MicroRNA target prediction and validation - p. 53 15. Chapter 5. Turning data to knowledge: online tools, databases, and resources in microRNA research - p. 69 16. Part IV: Molecular mechanisms and gene regulation - p. 85 17. Chapter 6. Function of microRNAs in the cytoplasm - p. 91 18. Chapter 7. MicroRNA turnover and nuclear function - p. 109 19. Chapter 8. MicroRNA-mediated transcriptional and posttranscriptional regulation - p. 141 20. Chapter 9. Epigenetic regulation and microRNA expression - p. 153 21. Chapter 10. RNA m6A modification and microRNAs - p. 169 22. Chapter 11. Unconventional functions of miRNAs - p. 181 23. Part V: MicroRNA as biomarkers - p. 202 24. Chapter 12. Detection methodologies for microRNA biomarker profiling - p. 217 25. Chapter 13. Endogenous circulating microRNAs in breast milk - p. 241 26. Part VI: MicroRNAs and human diseases - p. 250 27. Chapter 14. MicroRNAs and cancer - p. 253 28. Chapter 15. MicroRNAs and the immune system - p. 279 29. Chapter 16. MicroRNAs in cardiovascular diseases - p. 307 30. Chapter 17. MicroRNAs in neurological diseases - p. 317 31. Chapter 18. Role of miRNAs in muscle atrophy: the myotonic dystrophy paradigm - p. 331 32. Chapter 19. MiRNAs in liver fibrosis: new targets and opportunities for therapy - p. 363 33. Chapter 20. MicroRNAs and diabetes mellitus - p. 373 34. Chapter 21. MicroRNA control of kidney disease - p. 401 35. Chapter 22. MicroRNA in leukemia - p. 429 36. Chapter 23. MicroRNAs and other noncoding RNAs in human pathology - p. 469 37. Part VII: MicroRNA based therapeutics - p. 483 38. Chapter 24. Overview of microRNA-based therapeutics - p. 491 39. Chapter 25. MicroRNA-based therapeutic strategies for cancer - p. 503 40. Chapter 26. MicroRNA targeted therapy in cardiovascular disease - p. 521 41. Chapter 27. MicroRNA targeted therapy in hepatitis - p. 549 42. Chapter 28. MicroRNA-based therapeutic strategies for chronic kidney disease and uremic cardiomyopathy - p. 563 43. Chapter 29. Clinical applications of microRNAs - p. 601 44. Part VIII: Concluding perspectives - p. 609 45. Chapter 30. Prospective advances in microRNAs investigation - p. 613 46. Index - p. 625
MicroRNA: From Bench to Bedside provides an in-depth, expansive overview of microRNA from fundamentals to clinical practice. It presents researchers with detailed insights on the topic of microRNA, exploring foundational knowledge that is followed by methodologies and the latest technologies for research and potential theragnostic and therapeutic applications for specific diseases. The book consists of eight parts, beginning with an introduction to microRNA and the current state of the field, followed by sections on biogenesis and maturation of microRNA, and methodology and bioinformatics, where chapters focus on isolation and detection techniques.
Sections then move on to molecular mechanisms and gene regulation, considering topics such as transcriptional regulation and epigenetic regulation, as well as the role of microRNA as biomarkers. Additionally, microRNA and human disease and microRNA-based therapeutics are explored, focusing on a wide range of diseases such as cancer, age-related disease, cardiovascular disease, microRNA targeted therapy in hepatitis and therapeutic strategies for diabetes. The book concludes with a discussion on advances and future perspectives in microRNA investigation.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. xiii 7. Preface - p. xix 8. Part I: Overview - p. xx 9. Chapter 1. Overview of micro-RNA - p. 3 10. Part II: Biogenesis and maturation - p. 11 11. Chapter 2. Maturation of microRNAs - p. 19 12. Part III: Methodology and bioinformatics - p. 27 13. Chapter 3. MicroRNA interference - p. 33 14. Chapter 4. MicroRNA target prediction and validation - p. 53 15. Chapter 5. Turning data to knowledge: online tools, databases, and resources in microRNA research - p. 69 16. Part IV: Molecular mechanisms and gene regulation - p. 85 17. Chapter 6. Function of microRNAs in the cytoplasm - p. 91 18. Chapter 7. MicroRNA turnover and nuclear function - p. 109 19. Chapter 8. MicroRNA-mediated transcriptional and posttranscriptional regulation - p. 141 20. Chapter 9. Epigenetic regulation and microRNA expression - p. 153 21. Chapter 10. RNA m6A modification and microRNAs - p. 169 22. Chapter 11. Unconventional functions of miRNAs - p. 181 23. Part V: MicroRNA as biomarkers - p. 202 24. Chapter 12. Detection methodologies for microRNA biomarker profiling - p. 217 25. Chapter 13. Endogenous circulating microRNAs in breast milk - p. 241 26. Part VI: MicroRNAs and human diseases - p. 250 27. Chapter 14. MicroRNAs and cancer - p. 253 28. Chapter 15. MicroRNAs and the immune system - p. 279 29. Chapter 16. MicroRNAs in cardiovascular diseases - p. 307 30. Chapter 17. MicroRNAs in neurological diseases - p. 317 31. Chapter 18. Role of miRNAs in muscle atrophy: the myotonic dystrophy paradigm - p. 331 32. Chapter 19. MiRNAs in liver fibrosis: new targets and opportunities for therapy - p. 363 33. Chapter 20. MicroRNAs and diabetes mellitus - p. 373 34. Chapter 21. MicroRNA control of kidney disease - p. 401 35. Chapter 22. MicroRNA in leukemia - p. 429 36. Chapter 23. MicroRNAs and other noncoding RNAs in human pathology - p. 469 37. Part VII: MicroRNA based therapeutics - p. 483 38. Chapter 24. Overview of microRNA-based therapeutics - p. 491 39. Chapter 25. MicroRNA-based therapeutic strategies for cancer - p. 503 40. Chapter 26. MicroRNA targeted therapy in cardiovascular disease - p. 521 41. Chapter 27. MicroRNA targeted therapy in hepatitis - p. 549 42. Chapter 28. MicroRNA-based therapeutic strategies for chronic kidney disease and uremic cardiomyopathy - p. 563 43. Chapter 29. Clinical applications of microRNAs - p. 601 44. Part VIII: Concluding perspectives - p. 609 45. Chapter 30. Prospective advances in microRNAs investigation - p. 613 46. Index - p. 625
