Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 PART B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xv 7. Section 1 Structural studies of mononuclear non-hemeiron dependent enzymes - p. 1 8. Chapter One: Observing extradiol dioxygenases in action through a crystalline lens - p. 3 9. Chapter Two: Methods used to determine the structure of the oxygenase component of naphthalene 1,2 dioxygenase - p. 27 10. Chapter Three: Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: Biphenyl, Phthalate, and Terephthalate dioxygenases - p. 39 11. Chapter Four: Substitution of the mononuclear, non-heme iron cofactor in lipoxygenases for structural studies - p. 59 12. Section 2 Non-heme iron enzyme dependent reactions - p. 89 13. Chapter Five: Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxoglutarate dependent enzymes - p. 91 14. Chapter Six: Expression, purification and characterization of non-heme iron-dependent mono-oxygenase OzmD in oxazinomycin biosynthesis - p. 113 15. Chapter Seven: Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases - p. 143 16. Chapter Eight: Functional analysis of an α-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis - p. 173 17. Chapter Nine: Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ - p. 199 18. Chapter Ten: Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases - p. 233 19. Chapter Eleven: Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase - p. 259 20. Chapter Twelve: In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays - p. 291 21. Chapter Thirteen: Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine-β-hydroxylase - p. 313 22. Chapter Fourteen: Biochemical and biophysical approaches to characterization of the aromatic amino acid hydroxylases - p. 345
Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 PART B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xv 7. Section 1 Structural studies of mononuclear non-hemeiron dependent enzymes - p. 1 8. Chapter One: Observing extradiol dioxygenases in action through a crystalline lens - p. 3 9. Chapter Two: Methods used to determine the structure of the oxygenase component of naphthalene 1,2 dioxygenase - p. 27 10. Chapter Three: Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: Biphenyl, Phthalate, and Terephthalate dioxygenases - p. 39 11. Chapter Four: Substitution of the mononuclear, non-heme iron cofactor in lipoxygenases for structural studies - p. 59 12. Section 2 Non-heme iron enzyme dependent reactions - p. 89 13. Chapter Five: Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxoglutarate dependent enzymes - p. 91 14. Chapter Six: Expression, purification and characterization of non-heme iron-dependent mono-oxygenase OzmD in oxazinomycin biosynthesis - p. 113 15. Chapter Seven: Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases - p. 143 16. Chapter Eight: Functional analysis of an α-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis - p. 173 17. Chapter Nine: Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ - p. 199 18. Chapter Ten: Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases - p. 233 19. Chapter Eleven: Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase - p. 259 20. Chapter Twelve: In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays - p. 291 21. Chapter Thirteen: Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine-β-hydroxylase - p. 313 22. Chapter Fourteen: Biochemical and biophysical approaches to characterization of the aromatic amino acid hydroxylases - p. 345
