Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. Topics in this new release cover Enrichment/isolation, glycoproteomics, Mucinase cleavage characterization and usage in glycoproteomics, Fluorescent protein-based FRET substrates for activity screening of mucinases, Development of clinical lectin assays based on MS glycomic discovery, Enzymatic selective sulfation of synthetic mucins, cryoEM, Cell-based mucin arrays and glycoengineering, CRISPR screens to characterize and identify glycan-binding proteins, and much more.
Additional sections cover MD Simulations, Interactions with pathogenic bacteria, Interactions with microbiota, Covalent crosslinking of mucins, Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition, Quantification of mucins, Mucins in IBD, Investigations of mucins and innate immunity, Generating synthetic mucin glycopeptides, and Microscopy and imaging.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xix 8. Chapter One: Enrichment strategies for mucin-domain O-glycoproteins - p. 1 9. Chapter Two: Characterization of O-glycoproteases for applications in mass spectrometry - p. 31 10. Chapter Three: A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases - p. 65 11. Chapter Four: Clinical Europium fluorescent based lectin assays for mucin O-glycomics - p. 83 12. Chapter Five: Sialylation and fucosylation of mucin-type glycopeptides using recombinant glycosyltransferases - p. 117 13. Chapter Six: Cryo-electron microscopy of mucins - p. 159 14. Chapter Seven: Cell-based mucin arrays - p. 177 15. Chapter Eight: CRISPR screens to identify and characterize ligands for glycan-binding proteins - p. 219 16. Chapter Nine: Molecular dynamics simulations of mucins - p. 265 17. Chapter Ten: Methods for studying mucin–microbe interactions - p. 319 18. Chapter Eleven: Covalent crosslinking of mucins: From biomaterial design to tailored functions - p. 369 19. Chapter Twelve: Exploration of mucin-protein interactions using liquid glycan array - p. 391 20. Chapter Thirteen: Quantitative histological methods to define mucin alterations - p. 455 21. Chapter Fourteen: Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ - p. 475 22. Chapter Fifteen: Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues - p. 529 23. Chapter Sixteen: Ex vivo imaging and enzymatic analysis of intestinal mucus - p. 541 24. Chapter Seventeen: Evolutionary genetic approaches to analyze mucins - p. 569
Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. Topics in this new release cover Enrichment/isolation, glycoproteomics, Mucinase cleavage characterization and usage in glycoproteomics, Fluorescent protein-based FRET substrates for activity screening of mucinases, Development of clinical lectin assays based on MS glycomic discovery, Enzymatic selective sulfation of synthetic mucins, cryoEM, Cell-based mucin arrays and glycoengineering, CRISPR screens to characterize and identify glycan-binding proteins, and much more.
Additional sections cover MD Simulations, Interactions with pathogenic bacteria, Interactions with microbiota, Covalent crosslinking of mucins, Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition, Quantification of mucins, Mucins in IBD, Investigations of mucins and innate immunity, Generating synthetic mucin glycopeptides, and Microscopy and imaging.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xix 8. Chapter One: Enrichment strategies for mucin-domain O-glycoproteins - p. 1 9. Chapter Two: Characterization of O-glycoproteases for applications in mass spectrometry - p. 31 10. Chapter Three: A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases - p. 65 11. Chapter Four: Clinical Europium fluorescent based lectin assays for mucin O-glycomics - p. 83 12. Chapter Five: Sialylation and fucosylation of mucin-type glycopeptides using recombinant glycosyltransferases - p. 117 13. Chapter Six: Cryo-electron microscopy of mucins - p. 159 14. Chapter Seven: Cell-based mucin arrays - p. 177 15. Chapter Eight: CRISPR screens to identify and characterize ligands for glycan-binding proteins - p. 219 16. Chapter Nine: Molecular dynamics simulations of mucins - p. 265 17. Chapter Ten: Methods for studying mucin–microbe interactions - p. 319 18. Chapter Eleven: Covalent crosslinking of mucins: From biomaterial design to tailored functions - p. 369 19. Chapter Twelve: Exploration of mucin-protein interactions using liquid glycan array - p. 391 20. Chapter Thirteen: Quantitative histological methods to define mucin alterations - p. 455 21. Chapter Fourteen: Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ - p. 475 22. Chapter Fifteen: Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues - p. 529 23. Chapter Sixteen: Ex vivo imaging and enzymatic analysis of intestinal mucus - p. 541 24. Chapter Seventeen: Evolutionary genetic approaches to analyze mucins - p. 569
