Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.
Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter 1: Detection of myeloid-derived suppressor cells by flow cytometry - p. 1 8. Chapter 2: Flow cytometric detection of CD11b+ Gr-1+ cells in nontumor-bearing mice: A propolis-elicited model - p. 17 9. Chapter 3: Isolation of myeloid-derived suppressor cells (MDSC) from endometriotic mice model and their immunomodulatory functions - p. 33 10. Chapter 4: Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood - p. 59 11. Chapter 5: CD4+ T cells proliferation assay to analyze Mo-MDSCs suppressive function - p. 69 12. Chapter 6: Assessment of myeloid-derived suppressor cell differentiation ex vivo - p. 85 13. Chapter 7: Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo - p. 97 14. Chapter 8: Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes - p. 105 15. Chapter 9: Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells - p. 119 16. Chapter 10: In vitro osteoclastogenesis assessment using murine myeloid-derived suppressor cells - p. 133 17. Chapter 11: Estimating nitric oxide (NO) from MDSCs by Griess method - p. 149 18. Chapter 12: In vitro generation of murine myeloid-derived suppressor cells from hematopoietic progenitor cells - p. 159
Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.
Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter 1: Detection of myeloid-derived suppressor cells by flow cytometry - p. 1 8. Chapter 2: Flow cytometric detection of CD11b+ Gr-1+ cells in nontumor-bearing mice: A propolis-elicited model - p. 17 9. Chapter 3: Isolation of myeloid-derived suppressor cells (MDSC) from endometriotic mice model and their immunomodulatory functions - p. 33 10. Chapter 4: Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood - p. 59 11. Chapter 5: CD4+ T cells proliferation assay to analyze Mo-MDSCs suppressive function - p. 69 12. Chapter 6: Assessment of myeloid-derived suppressor cell differentiation ex vivo - p. 85 13. Chapter 7: Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo - p. 97 14. Chapter 8: Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes - p. 105 15. Chapter 9: Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells - p. 119 16. Chapter 10: In vitro osteoclastogenesis assessment using murine myeloid-derived suppressor cells - p. 133 17. Chapter 11: Estimating nitric oxide (NO) from MDSCs by Griess method - p. 149 18. Chapter 12: In vitro generation of murine myeloid-derived suppressor cells from hematopoietic progenitor cells - p. 159
