New Experimental Probes for Enzyme Specificity and Mechanism, Volume 685, the latest release in the Methods of Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics including Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis, New Mechanistic Probes to Identify Novel Substrates for N-Myristoyltransferases, Phosphonate and a Fluorophosphonate Analogues of D Glucose 6 Phosphate as Active-Site Probes of 1L-Myo-Inositol 1 phosphate Synthase, Kinetic Mechanism of Nicotine-Degrading Enzyme Probed by Stopped-Flow Kinetic Analyses, Kinetics and Mechanism for Enzyme-Catalyzed Reactions of Substrate Pieces, and more.
Additional chapters cover Kinetics and Mechanism for Reactions of Enzyme Pieces, Evaluation of allostery for the bienzyme assembly of a 3-deoxy-D-arabino heptulosonate-7-phosphate synthase and chorismate mutase, Recognition and Catalysis of Reactions of Chiral Substrates by Mandelate Racemase, Innovative and emerging modalities of EGFR kinase inhibitors, Characterization of the Aminoacrylate Intermediate of Tyrosine Phenol-Lyases, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Chapter One: Paracatalytic induction: Subverting specificity in hedgehog protein autoprocessing with small molecules - p. 1 9. Chapter Two: Identification of potential HDAC11 deacylase substrates by affinity pulldown MS - p. 43 10. Chapter Three: Phosphonate and α-fluorophosphonate analogs of D-glucose 6-phosphate as active-site probes of 1L-myo-inositol 1-phosphate synthase - p. 57 11. Chapter Four: Kinetics and mechanism for enzyme-catalyzed reactions of substrate pieces - p. 95 12. Chapter Five: Application of circular dichroism-based assays to racemases and epimerases: Recognition and catalysis of reactions of chiral substrates by mandelate racemase - p. 137 13. Chapter Six: Structural elements that enable specificity for mutant EGFR kinase domains with next-generation small-molecule inhibitors - p. 176 14. Chapter Seven: Characterization of aminoacrylate intermediates of pyridoxal-5′-phosphate dependent enzymes - p. 200 15. Chapter Eight: Employing deuterium kinetic isotope effects to uncover the mechanism of (R)-3-hydroxybutyrate dehydrogenase - p. 232 16. Chapter Nine: Probing the mechanism of flavin action in the oxidative decarboxylation catalyzed by salicylate hydroxylase - p. 241 17. Chapter Ten: Kinetic characterization of methylthio-D-ribose-1-phosphate isomerase - p. 279 18. Chapter Eleven: Atomistic description of the relationship between protein dynamics and catalysis with transition path sampling - p. 319 19. Chapter Twelve: The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis - p. 354 20. Chapter Thirteen: The unusual chemical sequences of mammalian dihydropyrimidine dehydrogenase revealed by transient-state analysis - p. 373 21. Chapter Fourteen: Combined spectroscopic and structural approaches to explore the mechanism of histidine-ligated heme-dependent aromatic oxygenases - p. 405 22. Chapter Fifteen: Biochemical methods to map and quantify allosteric motions in human glucokinase - p. 433 23. Chapter Sixteen: Design and interpretation of experiments to establish enzyme pathway and define the role of conformational changes in enzyme specificity - p. 461 24. Chapter Seventeen: Probing mechanistic questions in the PLP- and O2-dependent L-Arg oxidases - p. 493 25. Chapter Eighteen: Spectral deconvolution of electron-bifurcating flavoproteins - p. 531
New Experimental Probes for Enzyme Specificity and Mechanism, Volume 685, the latest release in the Methods of Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics including Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis, New Mechanistic Probes to Identify Novel Substrates for N-Myristoyltransferases, Phosphonate and a Fluorophosphonate Analogues of D Glucose 6 Phosphate as Active-Site Probes of 1L-Myo-Inositol 1 phosphate Synthase, Kinetic Mechanism of Nicotine-Degrading Enzyme Probed by Stopped-Flow Kinetic Analyses, Kinetics and Mechanism for Enzyme-Catalyzed Reactions of Substrate Pieces, and more.
Additional chapters cover Kinetics and Mechanism for Reactions of Enzyme Pieces, Evaluation of allostery for the bienzyme assembly of a 3-deoxy-D-arabino heptulosonate-7-phosphate synthase and chorismate mutase, Recognition and Catalysis of Reactions of Chiral Substrates by Mandelate Racemase, Innovative and emerging modalities of EGFR kinase inhibitors, Characterization of the Aminoacrylate Intermediate of Tyrosine Phenol-Lyases, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Chapter One: Paracatalytic induction: Subverting specificity in hedgehog protein autoprocessing with small molecules - p. 1 9. Chapter Two: Identification of potential HDAC11 deacylase substrates by affinity pulldown MS - p. 43 10. Chapter Three: Phosphonate and α-fluorophosphonate analogs of D-glucose 6-phosphate as active-site probes of 1L-myo-inositol 1-phosphate synthase - p. 57 11. Chapter Four: Kinetics and mechanism for enzyme-catalyzed reactions of substrate pieces - p. 95 12. Chapter Five: Application of circular dichroism-based assays to racemases and epimerases: Recognition and catalysis of reactions of chiral substrates by mandelate racemase - p. 137 13. Chapter Six: Structural elements that enable specificity for mutant EGFR kinase domains with next-generation small-molecule inhibitors - p. 176 14. Chapter Seven: Characterization of aminoacrylate intermediates of pyridoxal-5′-phosphate dependent enzymes - p. 200 15. Chapter Eight: Employing deuterium kinetic isotope effects to uncover the mechanism of (R)-3-hydroxybutyrate dehydrogenase - p. 232 16. Chapter Nine: Probing the mechanism of flavin action in the oxidative decarboxylation catalyzed by salicylate hydroxylase - p. 241 17. Chapter Ten: Kinetic characterization of methylthio-D-ribose-1-phosphate isomerase - p. 279 18. Chapter Eleven: Atomistic description of the relationship between protein dynamics and catalysis with transition path sampling - p. 319 19. Chapter Twelve: The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis - p. 354 20. Chapter Thirteen: The unusual chemical sequences of mammalian dihydropyrimidine dehydrogenase revealed by transient-state analysis - p. 373 21. Chapter Fourteen: Combined spectroscopic and structural approaches to explore the mechanism of histidine-ligated heme-dependent aromatic oxygenases - p. 405 22. Chapter Fifteen: Biochemical methods to map and quantify allosteric motions in human glucokinase - p. 433 23. Chapter Sixteen: Design and interpretation of experiments to establish enzyme pathway and define the role of conformational changes in enzyme specificity - p. 461 24. Chapter Seventeen: Probing mechanistic questions in the PLP- and O2-dependent L-Arg oxidases - p. 493 25. Chapter Eighteen: Spectral deconvolution of electron-bifurcating flavoproteins - p. 531
