New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. On, in, and under membrane - p. xi 8. Chapter One: Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell - p. 25 9. Chapter Two: Current methods for studying intracellular liquid-liquid phase separation - p. 55 10. Chapter Three: Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET - p. 75 11. Chapter Four: The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC - p. 119 12. Chapter Five: Studying cell volume beyond cell volume - p. 165 13. Chapter Six: Membrane tension - p. 189 14. Chapter Seven: Methods for assessment of membrane protrusion dynamics - p. 205 15. Chapter Eight: Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids - p. 235 16. Chapter Nine: Fluorescence sensors for imaging membrane lipid domains and cholesterol - p. 257 17. Chapter Ten: Mass spectrometry-based lipid analysis and imaging - p. 315 18. Chapter Eleven: Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering - p. 359
New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. On, in, and under membrane - p. xi 8. Chapter One: Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell - p. 25 9. Chapter Two: Current methods for studying intracellular liquid-liquid phase separation - p. 55 10. Chapter Three: Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET - p. 75 11. Chapter Four: The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC - p. 119 12. Chapter Five: Studying cell volume beyond cell volume - p. 165 13. Chapter Six: Membrane tension - p. 189 14. Chapter Seven: Methods for assessment of membrane protrusion dynamics - p. 205 15. Chapter Eight: Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids - p. 235 16. Chapter Nine: Fluorescence sensors for imaging membrane lipid domains and cholesterol - p. 257 17. Chapter Ten: Mass spectrometry-based lipid analysis and imaging - p. 315 18. Chapter Eleven: Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering - p. 359
