P450 Enzymes, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release cover Non-natural P450 reaction (carbene/nitrene), Non-natural P450 reaction (atom transfer radical cyclization), P450 fusion for biocatalysis, P450 fingerprinting for biocatalytic oxidation, Fungal peroxygenase, Fungal peroxygenase, Anti-Markovnikov P450 oxygenase (AmOX), Fungal P450, Unusual P450 chemistry from fungal sources, CASTing for P450 biocatalysis, and P450 in C–C coupling of cyclic dipeptides with nucleobases.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Directed evolution of P411 enzymes for amination of inert C–H bonds - p. 1 9. Chapter Two: P450-catalyzed atom transfer radical cyclization - p. 31 10. Chapter Three: Self-sufficient P450-reductase chimeras for biocatalysis - p. 51 11. Chapter Four: The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution - p. 73 12. Chapter Five: Engineering cytochrome P450s for selective alkene to carbonyl oxidation - p. 111 13. Chapter Six: Bacterial cytochrome P450 enzymes: Semi-rational design and screening of mutant libraries in recombinant Escherichia coli cells - p. 133 14. Chapter Seven: Functional analysis of a fungal P450 enzyme - p. 171 15. Chapter Eight: Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation - p. 191 16. Chapter Nine: P450 in C–C coupling of cyclodipeptides with nucleobases - p. 231 17. Chapter Ten: Secretion and directed evolution of unspecific peroxygenases in S. cerevisiae - p. 267 18. Chapter Eleven: Direct aromatic nitration by bacterial P450 enzymes - p. 307 19. Chapter Twelve: P450 fatty acid decarboxylase - p. 339 20. Chapter Thirteen: Directed evolution for Si–C bond cleavage of volatile siloxanes in glass bioreactors - p. 375
P450 Enzymes, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release cover Non-natural P450 reaction (carbene/nitrene), Non-natural P450 reaction (atom transfer radical cyclization), P450 fusion for biocatalysis, P450 fingerprinting for biocatalytic oxidation, Fungal peroxygenase, Fungal peroxygenase, Anti-Markovnikov P450 oxygenase (AmOX), Fungal P450, Unusual P450 chemistry from fungal sources, CASTing for P450 biocatalysis, and P450 in C–C coupling of cyclic dipeptides with nucleobases.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Directed evolution of P411 enzymes for amination of inert C–H bonds - p. 1 9. Chapter Two: P450-catalyzed atom transfer radical cyclization - p. 31 10. Chapter Three: Self-sufficient P450-reductase chimeras for biocatalysis - p. 51 11. Chapter Four: The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution - p. 73 12. Chapter Five: Engineering cytochrome P450s for selective alkene to carbonyl oxidation - p. 111 13. Chapter Six: Bacterial cytochrome P450 enzymes: Semi-rational design and screening of mutant libraries in recombinant Escherichia coli cells - p. 133 14. Chapter Seven: Functional analysis of a fungal P450 enzyme - p. 171 15. Chapter Eight: Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation - p. 191 16. Chapter Nine: P450 in C–C coupling of cyclodipeptides with nucleobases - p. 231 17. Chapter Ten: Secretion and directed evolution of unspecific peroxygenases in S. cerevisiae - p. 267 18. Chapter Eleven: Direct aromatic nitration by bacterial P450 enzymes - p. 307 19. Chapter Twelve: P450 fatty acid decarboxylase - p. 339 20. Chapter Thirteen: Directed evolution for Si–C bond cleavage of volatile siloxanes in glass bioreactors - p. 375
