Practical Approaches to Biological Inorganic Chemistry, Second Edition, reviews the use of spectroscopic and related analytical techniques to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each chapter presents an overview of the technique, including relevant theory, a clear explanation of what it is, how it works, and how the technique is actually used to evaluate biological structures. New chapters cover Raman Spectroscopy and Molecular Magnetochemistry, but all chapters have been updated to reflect the latest developments in discussed techniques. Practical examples, problems and many color figures are also included to illustrate key concepts.
The book is designed for researchers and students who want to learn both the basics and more advanced aspects of key methods in biological inorganic chemistry.
1. Chapter 2. Introduction to ligand field theory and computational chemistry - p. 17 2. Chapter 3. Molecular magnetochemistry - p. 69 3. Abstract - p. 69 4. Introduction - p. 69 5. Units and definitions - p. 77 6. Contributions to magnetism in biologically relevant ions - p. 92 7. Dimeric sites: exchange mechanisms and J values - p. 99 8. Diamagnetism - p. 105 9. Experimental methods - p. 108 10. Conclusion - p. 115 11. Problems - p. 115 12. Answers - p. 116 13. Acknowledgments - p. 119 14. References - p. 119 15. Further reading - p. 119 16. Chapter 4. EPR spectroscopy - p. 121 17. Chapter 5. Introduction to biomolecular nuclear magnetic resonance and metals - p. 155 18. Chapter 6. 57Fe-Mössbauer spectroscopy and basic interpretation of Mössbauer parameters - p. 201 19. Chapter 7. X-ray absorption and emission spectroscopy in biology - p. 229 20. Chapter 8. Resonance Raman spectroscopy and its application in bioinorganic chemistry - p. 275 21. Chapter 9. An introduction to electrochemical methods for the functional analysis of metalloproteins - p. 325 22. Chapter 10. Structural biology techniques: X-ray crystallography, cryo-electron microscopy, and small-angle X-ray scattering - p. 375 23. Chapter 11. Genetic and molecular biological approaches for the study of metals in biology - p. 417 24. Index - p. 477
Practical Approaches to Biological Inorganic Chemistry, Second Edition, reviews the use of spectroscopic and related analytical techniques to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each chapter presents an overview of the technique, including relevant theory, a clear explanation of what it is, how it works, and how the technique is actually used to evaluate biological structures. New chapters cover Raman Spectroscopy and Molecular Magnetochemistry, but all chapters have been updated to reflect the latest developments in discussed techniques. Practical examples, problems and many color figures are also included to illustrate key concepts.
The book is designed for researchers and students who want to learn both the basics and more advanced aspects of key methods in biological inorganic chemistry.
1. Chapter 2. Introduction to ligand field theory and computational chemistry - p. 17 2. Chapter 3. Molecular magnetochemistry - p. 69 3. Abstract - p. 69 4. Introduction - p. 69 5. Units and definitions - p. 77 6. Contributions to magnetism in biologically relevant ions - p. 92 7. Dimeric sites: exchange mechanisms and J values - p. 99 8. Diamagnetism - p. 105 9. Experimental methods - p. 108 10. Conclusion - p. 115 11. Problems - p. 115 12. Answers - p. 116 13. Acknowledgments - p. 119 14. References - p. 119 15. Further reading - p. 119 16. Chapter 4. EPR spectroscopy - p. 121 17. Chapter 5. Introduction to biomolecular nuclear magnetic resonance and metals - p. 155 18. Chapter 6. 57Fe-Mössbauer spectroscopy and basic interpretation of Mössbauer parameters - p. 201 19. Chapter 7. X-ray absorption and emission spectroscopy in biology - p. 229 20. Chapter 8. Resonance Raman spectroscopy and its application in bioinorganic chemistry - p. 275 21. Chapter 9. An introduction to electrochemical methods for the functional analysis of metalloproteins - p. 325 22. Chapter 10. Structural biology techniques: X-ray crystallography, cryo-electron microscopy, and small-angle X-ray scattering - p. 375 23. Chapter 11. Genetic and molecular biological approaches for the study of metals in biology - p. 417 24. Index - p. 477
