Proteomics in Cell Communication, offers a comprehensive exploration of the dynamic field of proteomics and its vital role in understanding cellular communication. This book includes interesting chapters on topics such as In vivo cell-type specific secretome profiling, Assays for surface antigens in extracellular vesicles using proximity labeling strategy, Cell type-specific proximity biotinylation using TurboID to identify secreted proteins from cardiomyocytes in vitro and in vivo, Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models, Adapted Luminex approach to assess phosphoproteins and cytokines released from specific cell types in the brain in vivo, and much more.
Other sections covers In-depth cell-selective proteomics and secretomics in vivo based on azidonorleucine labelling, Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity, A Split GFP Approach for Cell-Type-Specific Protein Localization Analysis in Drosophila, Quantitative deep profiling of the cell surface proteome using the "Cell Surface Capture" technique, Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues, and much more.
1. Proteomics in Cell Communication - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. xi 8. Foreword - p. xv 9. Chapter One In vivo cell-type specific secretome profiling - p. 1 10. Chapter Two Assays for surface antigens in extracellular vesicles using proximity labeling strategy - p. 22 11. Chapter Three Murine adult cardiomyocytes isolation and AAV transduction for secretome profiling using proximity labeling - p. 49 12. Chapter Four Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models - p. 61 13. Chapter Five Direct quantification of cell type-specific proteins using Luminex assays with TurboID-labeled cells and tissues - p. 90 14. Chapter Six MetRS*-based deep cell-selective tissue proteomics and secretomics in vivo - p. 105 15. Chapter Seven Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity - p. 120 16. Chapter Eight Cell-type-specific labeling of endogenous proteins using the split GFP system in Drosophila - p. 138 17. Chapter Nine Enhanced cell surface enrichment and data-independent acquisition for in-depth profiling of multiple myeloma cell lines - p. 151 18. Chapter Ten Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues - p. 172 19. Chapter Eleven A cell culture-based method for interrogating muscle to liver communication via secreted proteins - p. 197 20. Chapter Twelve SILAC-based assessment of S-palmitoylated proteins in mammalian cells by metabolic labeling and click-chemistry - p. 213 21. Chapter Thirteen Separating the wheat from the chaff: Determination of confidently secreted proteins by including information on their cellular abundance - p. 245
Proteomics in Cell Communication, offers a comprehensive exploration of the dynamic field of proteomics and its vital role in understanding cellular communication. This book includes interesting chapters on topics such as In vivo cell-type specific secretome profiling, Assays for surface antigens in extracellular vesicles using proximity labeling strategy, Cell type-specific proximity biotinylation using TurboID to identify secreted proteins from cardiomyocytes in vitro and in vivo, Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models, Adapted Luminex approach to assess phosphoproteins and cytokines released from specific cell types in the brain in vivo, and much more.
Other sections covers In-depth cell-selective proteomics and secretomics in vivo based on azidonorleucine labelling, Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity, A Split GFP Approach for Cell-Type-Specific Protein Localization Analysis in Drosophila, Quantitative deep profiling of the cell surface proteome using the "Cell Surface Capture" technique, Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues, and much more.
1. Proteomics in Cell Communication - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. xi 8. Foreword - p. xv 9. Chapter One In vivo cell-type specific secretome profiling - p. 1 10. Chapter Two Assays for surface antigens in extracellular vesicles using proximity labeling strategy - p. 22 11. Chapter Three Murine adult cardiomyocytes isolation and AAV transduction for secretome profiling using proximity labeling - p. 49 12. Chapter Four Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models - p. 61 13. Chapter Five Direct quantification of cell type-specific proteins using Luminex assays with TurboID-labeled cells and tissues - p. 90 14. Chapter Six MetRS*-based deep cell-selective tissue proteomics and secretomics in vivo - p. 105 15. Chapter Seven Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity - p. 120 16. Chapter Eight Cell-type-specific labeling of endogenous proteins using the split GFP system in Drosophila - p. 138 17. Chapter Nine Enhanced cell surface enrichment and data-independent acquisition for in-depth profiling of multiple myeloma cell lines - p. 151 18. Chapter Ten Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues - p. 172 19. Chapter Eleven A cell culture-based method for interrogating muscle to liver communication via secreted proteins - p. 197 20. Chapter Twelve SILAC-based assessment of S-palmitoylated proteins in mammalian cells by metabolic labeling and click-chemistry - p. 213 21. Chapter Thirteen Separating the wheat from the chaff: Determination of confidently secreted proteins by including information on their cellular abundance - p. 245
