Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more.
Other chapters cover Methods to study FOXP3 CD8 cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series page - p. iii 5. Copyright page - p. iii 6. Contributors - p. iii 7. Radiation therapy: An old dog learning new tricks - p. iii 8. Chapter 1: Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice - p. 1 9. Chapter 2: Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors - p. 17 10. CHAPTER 3: Mass cytometry to characterize the immune lung cancer microenvironment - p. 31 11. Chapter 4: Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence - p. 43 12. Chapter 5: Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants - p. 55 13. Chapter 6: Assessment of immunological memory formation in vivo - p. 65 14. Chapter 7: MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia - p. 75 15. Chapter 8: Quantitative assessment of mitophagy in irradiated cancer cells - p. 93 16. Chapter 9: Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence - p. 113 17. Chapter 10: A simple method to assess clonogenic survival of irradiated cancer cells - p. 126 18. Chapter 11: Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy - p. 137
Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more.
Other chapters cover Methods to study FOXP3 CD8 cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series page - p. iii 5. Copyright page - p. iii 6. Contributors - p. iii 7. Radiation therapy: An old dog learning new tricks - p. iii 8. Chapter 1: Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice - p. 1 9. Chapter 2: Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors - p. 17 10. CHAPTER 3: Mass cytometry to characterize the immune lung cancer microenvironment - p. 31 11. Chapter 4: Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence - p. 43 12. Chapter 5: Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants - p. 55 13. Chapter 6: Assessment of immunological memory formation in vivo - p. 65 14. Chapter 7: MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia - p. 75 15. Chapter 8: Quantitative assessment of mitophagy in irradiated cancer cells - p. 93 16. Chapter 9: Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence - p. 113 17. Chapter 10: A simple method to assess clonogenic survival of irradiated cancer cells - p. 126 18. Chapter 11: Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy - p. 137
