Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more.
Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xiii 8. Chapter One: Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules - p. 1 9. Chapter Two: Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations - p. 23 10. Chapter Three: Electron paramagnetic resonance of lanthanides - p. 71 11. Chapter Four: Characterization of lanthanoid-binding proteins using NMR spectroscopy - p. 127 12. Chapter Five: Macromolecular crystallography for f-element complex characterization - p. 139 13. Chapter Six: Infrared spectroscopy probes ion binding geometries - p. 157 14. Chapter Seven: Predicting lanthanide coordination structures in solution with molecular simulation - p. 201 15. Chapter Eight: Characteristics of Gd(III) spin labels for the study of protein conformations - p. 235 16. Chapter Nine: Lanthanide-based resonance energy transfer biosensors for live-cell applications - p. 291 17. Chapter Ten: 86Y PET imaging - p. 320 18. Chapter Eleven: Aqueous chemistry of the smallest rare earth: Comprehensive characterization of radioactive and non-radioactive scandium complexes for biological applications - p. 343 19. Chapter Twelve: In vitro selection and application of lanthanide-dependent DNAzymes - p. 373
Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more.
Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xiii 8. Chapter One: Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules - p. 1 9. Chapter Two: Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations - p. 23 10. Chapter Three: Electron paramagnetic resonance of lanthanides - p. 71 11. Chapter Four: Characterization of lanthanoid-binding proteins using NMR spectroscopy - p. 127 12. Chapter Five: Macromolecular crystallography for f-element complex characterization - p. 139 13. Chapter Six: Infrared spectroscopy probes ion binding geometries - p. 157 14. Chapter Seven: Predicting lanthanide coordination structures in solution with molecular simulation - p. 201 15. Chapter Eight: Characteristics of Gd(III) spin labels for the study of protein conformations - p. 235 16. Chapter Nine: Lanthanide-based resonance energy transfer biosensors for live-cell applications - p. 291 17. Chapter Ten: 86Y PET imaging - p. 320 18. Chapter Eleven: Aqueous chemistry of the smallest rare earth: Comprehensive characterization of radioactive and non-radioactive scandium complexes for biological applications - p. 343 19. Chapter Twelve: In vitro selection and application of lanthanide-dependent DNAzymes - p. 373
