Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo.
Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications.
1. Chapter 1. Introduction - p. 1 2. Chapter 2. Chemistry of Reactive Species - p. 13 3. Abstract - p. 13 4. 2.1 Introduction - p. 13 5. 2.2 Redox Chemistry - p. 14 6. 2.3 Properties of Reactive Species - p. 15 7. References - p. 54 8. Chapter 3. Reactive Species in Biological Systems - p. 65 9. Chapter 4. Fluorescence Technique - p. 87 10. Chapter 5. EPR Spin Trapping - p. 163 11. Chapter 6. UV–Vis Absorption and Chemiluminescence Techniques - p. 203 12. Chapter 7. Electrochemical, Mass Spectroscopic, Immunochemical, and Nuclear Magnetic Resonance Techniques - p. 253 13. Index - p. 323
Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo.
Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications.
1. Chapter 1. Introduction - p. 1 2. Chapter 2. Chemistry of Reactive Species - p. 13 3. Abstract - p. 13 4. 2.1 Introduction - p. 13 5. 2.2 Redox Chemistry - p. 14 6. 2.3 Properties of Reactive Species - p. 15 7. References - p. 54 8. Chapter 3. Reactive Species in Biological Systems - p. 65 9. Chapter 4. Fluorescence Technique - p. 87 10. Chapter 5. EPR Spin Trapping - p. 163 11. Chapter 6. UV–Vis Absorption and Chemiluminescence Techniques - p. 203 12. Chapter 7. Electrochemical, Mass Spectroscopic, Immunochemical, and Nuclear Magnetic Resonance Techniques - p. 253 13. Index - p. 323