Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Section I: Yeast hosts - p. xxii 9. Chapter One: Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae - p. 3 10. Chapter Two: Recombinant production of membrane proteins in yeast - p. 21 11. Chapter Three: Expression of proteins in Pichia pastoris - p. 53 12. Chapter Four: Hybrid-architectured promoter design to engineer expression in yeast - p. 81 13. Chapter Five: Hybrid-architectured promoter design to deregulate expression in yeast - p. 105 14. Section II: Baculovirus–insect cell systems - p. 125 15. Chapter Six: The MultiBac BEVS: Basics, applications, performance and recent developments - p. 129 16. Chapter Seven: Applications of Golden Gate cloning to protein production using the baculovirus expression vector system - p. 155 17. Chapter Eight: A fast-track protocol for protein expression using the BEV system - p. 171 18. Section III: Plant hosts - p. 190 19. Chapter Nine: Transient expression of recombinant proteins in plants - p. 193 20. Chapter Ten: Transient expression of recombinant proteins in plants using potato virus X based vectors - p. 205 21. Chapter Eleven: Design and expression of a bispecific antibody against dengue and chikungunya virus in plants - p. 223 22. Chapter Twelve: Manufacturing plant-made monoclonal antibodies for research or therapeutic applications - p. 257 23. Section IV: Mammalian hosts - p. 263 24. Chapter Thirteen: Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation - p. 289 25. Chapter Fourteen: Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors - p. 297 26. Chapter Fifteen: Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools - p. 321 27. Chapter Sixteen: Exploring the design space of AAV transient-transfection in suspension cell lines - p. 341
Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xvii 8. Section I: Yeast hosts - p. xxii 9. Chapter One: Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae - p. 3 10. Chapter Two: Recombinant production of membrane proteins in yeast - p. 21 11. Chapter Three: Expression of proteins in Pichia pastoris - p. 53 12. Chapter Four: Hybrid-architectured promoter design to engineer expression in yeast - p. 81 13. Chapter Five: Hybrid-architectured promoter design to deregulate expression in yeast - p. 105 14. Section II: Baculovirus–insect cell systems - p. 125 15. Chapter Six: The MultiBac BEVS: Basics, applications, performance and recent developments - p. 129 16. Chapter Seven: Applications of Golden Gate cloning to protein production using the baculovirus expression vector system - p. 155 17. Chapter Eight: A fast-track protocol for protein expression using the BEV system - p. 171 18. Section III: Plant hosts - p. 190 19. Chapter Nine: Transient expression of recombinant proteins in plants - p. 193 20. Chapter Ten: Transient expression of recombinant proteins in plants using potato virus X based vectors - p. 205 21. Chapter Eleven: Design and expression of a bispecific antibody against dengue and chikungunya virus in plants - p. 223 22. Chapter Twelve: Manufacturing plant-made monoclonal antibodies for research or therapeutic applications - p. 257 23. Section IV: Mammalian hosts - p. 263 24. Chapter Thirteen: Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation - p. 289 25. Chapter Fourteen: Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors - p. 297 26. Chapter Fifteen: Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools - p. 321 27. Chapter Sixteen: Exploring the design space of AAV transient-transfection in suspension cell lines - p. 341
