Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Copyright - p. iii 4. Contributors - p. iii 5. Preface - p. xi 6. Chapter One: Development of insect cell line using CRISPR technology - p. 1 7. Chapter Two: CRISPRing protozoan parasites to better understand the biology of diseases - p. 21 8. Chapter Three: CRISPR-based genome editing of zebrafish - p. 69 9. Chapter Four: Using CRISPR-Cas9-based genome engineering tools in Drosophila melanogaster - p. 95 10. Chapter Five: CRISPR interference and its applications - p. 123 11. Chapter Six: CRISPR-Cas systems: Challenges and future prospects - p. 145 12. Chapter Seven: Patents and technology transfer in CRISPR technology - p. 161 13. Index - p. 182
Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Copyright - p. iii 4. Contributors - p. iii 5. Preface - p. xi 6. Chapter One: Development of insect cell line using CRISPR technology - p. 1 7. Chapter Two: CRISPRing protozoan parasites to better understand the biology of diseases - p. 21 8. Chapter Three: CRISPR-based genome editing of zebrafish - p. 69 9. Chapter Four: Using CRISPR-Cas9-based genome engineering tools in Drosophila melanogaster - p. 95 10. Chapter Five: CRISPR interference and its applications - p. 123 11. Chapter Six: CRISPR-Cas systems: Challenges and future prospects - p. 145 12. Chapter Seven: Patents and technology transfer in CRISPR technology - p. 161 13. Index - p. 182
