Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms enables researchers in bioscience to visualize cellular phenomena at an unprecedented scale and rate. The book's editors provide an overview of each of the most current techniques, both individually and when used in conjunction. Sections cover CyTof, Multicolor fluorescence cytometry, CODEX multiplexed imaging, MIBI, Imaging Mass Cytometry, Multi resolution cell orientation, Confocal microscopy, QPI and Fluorescence microscopy, CyTOF and SCRNA Seq, CyTOP and scATAC Seq, and CyTOF and ATAC See, and the application of the techniques to biological questions, including chapters on cellular diversity and generating hypothesis through data analysis. The final part is dedicated to further the reader’s understanding of complex biological systems by identifying new clinical tools and drug development. This book is the ideal reference for researchers who want to understand the variety of multiplex visualization techniques available for bioscientists and how to make better decisions on how to apply them to the biological question of interest.
1. Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Dedication - p. Page v 7. Contributors - p. Page v 8. Foreword - p. Page vii 9. Acknowledgments - p. Page ix 10. List of Illustrations - p. Page x 11. List of Tables - p. Page xi 12. Chapter 1 : Personal and historical recollections of the early years of mass cytometry - p. Page xi 13. Chapter 2 : Unveiling spatial biology of cellular landscapes with imaging mass cytometry - p. Page 20 14. Chapter 3 : Single-cell spatial phenotype mapping of tissue microenvironments with multiplexed ion beam imaging (MIBI): A highly multiplexed, high-resolution spatial proteomic approach for tissue imaging and analysis - p. Page 53 15. Chapter 4 : Highly multiplexed spatial protein data using CODEX technology - p. Page 88 16. Chapter 5 : Exploring uncharted spatial biology with ChipCytometry highly multiplexed imaging - p. Page 116 17. Chapter 6 : Combined nucleic acid and protein quantification in the native tissue microenvironment - p. Page 144 18. Chapter 7 : Tissue schematics: Representing tissues as assemblies of neighborhoods - p. Page 161 19. Chapter 8 : High-definition CODEX for 3D multiplex spatial cell phenotyping - p. Page 187 20. Index - p. Page 212
Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms enables researchers in bioscience to visualize cellular phenomena at an unprecedented scale and rate. The book's editors provide an overview of each of the most current techniques, both individually and when used in conjunction. Sections cover CyTof, Multicolor fluorescence cytometry, CODEX multiplexed imaging, MIBI, Imaging Mass Cytometry, Multi resolution cell orientation, Confocal microscopy, QPI and Fluorescence microscopy, CyTOF and SCRNA Seq, CyTOP and scATAC Seq, and CyTOF and ATAC See, and the application of the techniques to biological questions, including chapters on cellular diversity and generating hypothesis through data analysis. The final part is dedicated to further the reader’s understanding of complex biological systems by identifying new clinical tools and drug development. This book is the ideal reference for researchers who want to understand the variety of multiplex visualization techniques available for bioscientists and how to make better decisions on how to apply them to the biological question of interest.
1. Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Dedication - p. Page v 7. Contributors - p. Page v 8. Foreword - p. Page vii 9. Acknowledgments - p. Page ix 10. List of Illustrations - p. Page x 11. List of Tables - p. Page xi 12. Chapter 1 : Personal and historical recollections of the early years of mass cytometry - p. Page xi 13. Chapter 2 : Unveiling spatial biology of cellular landscapes with imaging mass cytometry - p. Page 20 14. Chapter 3 : Single-cell spatial phenotype mapping of tissue microenvironments with multiplexed ion beam imaging (MIBI): A highly multiplexed, high-resolution spatial proteomic approach for tissue imaging and analysis - p. Page 53 15. Chapter 4 : Highly multiplexed spatial protein data using CODEX technology - p. Page 88 16. Chapter 5 : Exploring uncharted spatial biology with ChipCytometry highly multiplexed imaging - p. Page 116 17. Chapter 6 : Combined nucleic acid and protein quantification in the native tissue microenvironment - p. Page 144 18. Chapter 7 : Tissue schematics: Representing tissues as assemblies of neighborhoods - p. Page 161 19. Chapter 8 : High-definition CODEX for 3D multiplex spatial cell phenotyping - p. Page 187 20. Index - p. Page 212
