RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise.
1. Cover image - p. Cover 2. Title page - p. iii 3. Table of Contents - p. iii 4. Dedication - p. ii 5. Copyright - p. iv 6. Preface - p. xvii 7. Chapter 1. RNA and the Cellular Biochemistry Revisited - p. 1 8. Chapter 2. Creating a Ribonuclease-Free Environment - p. 55 9. Chapter 3. RNA Isolation Strategies - p. 75 10. Chapter 4. The Truth About Tissues - p. 117 11. Chapter 5. Going Green: RNA and the Molecular Biology of Plants - p. 145 12. Chapter 6. Quality Control for RNA Preparations - p. 167 13. Chapter 7. cDNA—A Permanent Biochemical Record of the Cell - p. 187 14. Chapter 8. RT-PCR: A Science and an Art Form - p. 209 15. Chapter 9. Quantitative PCR Techniques - p. 283 16. Chapter 10. miRNA - p. 329 17. Chapter 11. RNA Interference and RNA Editing - p. 345 18. Chapter 12. Stringency: Conditions That Influence Nucleic Acid Structure - p. 377 19. Chapter 13. Electrophoresis of RNA - p. 383 20. Chapter 14. Photodocumentation and Image Analysis - p. 427 21. Chapter 15. Northern Analysis - p. 447 22. Chapter 16. Nucleic Acid Probe Technology - p. 469 23. Chapter 17. Isolation of Polyadenylated RNA - p. 527 24. Chapter 18. Quantification of Specific mRNAs by Nuclease Protection - p. 545 25. Chapter 19. Analysis of Nuclear RNA - p. 569 26. Chapter 20. Nonarray Methods for Global Analysis of Gene Expression - p. 601 27. Chapter 21. Genomes, Transcriptomes, Proteomes, and Bioinformatics - p. 629 28. Chapter 22. RNA Biomarker Discovery - p. 655 29. Chapter 23. High-Throughput Analysis of Gene Expression - p. 667 30. Chapter 24. Functional Genomics and Transcript Profiling - p. 685 31. Chapter 25. A Few RNA Success Stories - p. 697 32. Epilogue: A Few Pearls of Wisdom - p. 707 33. Appendix A. Maintaining Complete and Accurate Records - p. 715 34. Appendix B. Converting Mass to Moles - p. 719 35. Appendix C. Disposal of Ethidium Bromide and SYBR Green Solutions - p. 723 36. Appendix D. Removal of DNA From an RNA Sample - p. 727 37. Appendix E. Removal of RNA From a DNA Sample - p. 729 38. Appendix F. Useful Stock Solutions for the Molecular Biologist - p. 731 39. Appendix G. Silanizing Centrifuge Tubes and Glassware - p. 737 40. Appendix H. Dot Blot Analysis - p. 739 41. Appendix I. Electrophoresis: Principles, Parameters, and Safety - p. 749 42. Appendix J. Polyacrylamide Gel Electrophoresis - p. 761 43. Appendix K. Centrifugation as a Mainstream Tool for the Molecular Biologist - p. 765 44. Appendix L. Trypsinization Protocol for Anchorage-Dependent Cells - p. 773 45. Appendix M. Isolation of High-Molecular-Weight DNA by Salting-Out - p. 777 46. Appendix N. Phenol Preparation - p. 781 47. Appendix O. Deionization of Formamide, Formaldehyde, and Glyoxal - p. 785 48. Appendix P. Selected Suppliers of Equipment, Reagents, and Services - p. 787 49. Appendix Q. Useful SI Prefixes and Common SI Base Units - p. 797 50. Appendix R. Common Abbreviations - p. 799 51. Appendix S. Trademark Citations - p. 801 52. Glossary - p. 805 53. Index - p. 833
RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise.
1. Cover image - p. Cover 2. Title page - p. iii 3. Table of Contents - p. iii 4. Dedication - p. ii 5. Copyright - p. iv 6. Preface - p. xvii 7. Chapter 1. RNA and the Cellular Biochemistry Revisited - p. 1 8. Chapter 2. Creating a Ribonuclease-Free Environment - p. 55 9. Chapter 3. RNA Isolation Strategies - p. 75 10. Chapter 4. The Truth About Tissues - p. 117 11. Chapter 5. Going Green: RNA and the Molecular Biology of Plants - p. 145 12. Chapter 6. Quality Control for RNA Preparations - p. 167 13. Chapter 7. cDNA—A Permanent Biochemical Record of the Cell - p. 187 14. Chapter 8. RT-PCR: A Science and an Art Form - p. 209 15. Chapter 9. Quantitative PCR Techniques - p. 283 16. Chapter 10. miRNA - p. 329 17. Chapter 11. RNA Interference and RNA Editing - p. 345 18. Chapter 12. Stringency: Conditions That Influence Nucleic Acid Structure - p. 377 19. Chapter 13. Electrophoresis of RNA - p. 383 20. Chapter 14. Photodocumentation and Image Analysis - p. 427 21. Chapter 15. Northern Analysis - p. 447 22. Chapter 16. Nucleic Acid Probe Technology - p. 469 23. Chapter 17. Isolation of Polyadenylated RNA - p. 527 24. Chapter 18. Quantification of Specific mRNAs by Nuclease Protection - p. 545 25. Chapter 19. Analysis of Nuclear RNA - p. 569 26. Chapter 20. Nonarray Methods for Global Analysis of Gene Expression - p. 601 27. Chapter 21. Genomes, Transcriptomes, Proteomes, and Bioinformatics - p. 629 28. Chapter 22. RNA Biomarker Discovery - p. 655 29. Chapter 23. High-Throughput Analysis of Gene Expression - p. 667 30. Chapter 24. Functional Genomics and Transcript Profiling - p. 685 31. Chapter 25. A Few RNA Success Stories - p. 697 32. Epilogue: A Few Pearls of Wisdom - p. 707 33. Appendix A. Maintaining Complete and Accurate Records - p. 715 34. Appendix B. Converting Mass to Moles - p. 719 35. Appendix C. Disposal of Ethidium Bromide and SYBR Green Solutions - p. 723 36. Appendix D. Removal of DNA From an RNA Sample - p. 727 37. Appendix E. Removal of RNA From a DNA Sample - p. 729 38. Appendix F. Useful Stock Solutions for the Molecular Biologist - p. 731 39. Appendix G. Silanizing Centrifuge Tubes and Glassware - p. 737 40. Appendix H. Dot Blot Analysis - p. 739 41. Appendix I. Electrophoresis: Principles, Parameters, and Safety - p. 749 42. Appendix J. Polyacrylamide Gel Electrophoresis - p. 761 43. Appendix K. Centrifugation as a Mainstream Tool for the Molecular Biologist - p. 765 44. Appendix L. Trypsinization Protocol for Anchorage-Dependent Cells - p. 773 45. Appendix M. Isolation of High-Molecular-Weight DNA by Salting-Out - p. 777 46. Appendix N. Phenol Preparation - p. 781 47. Appendix O. Deionization of Formamide, Formaldehyde, and Glyoxal - p. 785 48. Appendix P. Selected Suppliers of Equipment, Reagents, and Services - p. 787 49. Appendix Q. Useful SI Prefixes and Common SI Base Units - p. 797 50. Appendix R. Common Abbreviations - p. 799 51. Appendix S. Trademark Citations - p. 801 52. Glossary - p. 805 53. Index - p. 833
