Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases - p. xviii 8. Chapter Two: Single-Molecule Imaging With One Color Fluorescence - p. 32 9. Chapter Three: Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy - p. 51 10. Chapter Four: Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging - p. 82 11. Chapter Five: Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy - p. 104 12. Chapter Six: How to Measure Separations and Angles Between Intramolecular Fluorescent Markers - p. 145 13. Chapter Seven: Precisely and Accurately Inferring Single-Molecule Rate Constants - p. 185 14. Chapter Eight: Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout - p. 225 15. Chapter Nine: Putting Humpty–Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome - p. 256 16. Chapter Ten: Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins - p. 283 17. Chapter Eleven: Single-Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases - p. 315 18. Chapter Twelve: Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET - p. 351 19. Chapter Thirteen: Methods for Investigating DNA Accessibility with Single Nucleosomes - p. 378 20. Chapter Fourteen: Single-Molecule Fluorescence Studies of Fast Protein Folding - p. 415 21. Chapter Fifteen: Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules - p. 459 22. Chapter Sixteen: A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously - p. 486 23. Chapter Seventeen: Interferometric Scattering Microscopy for the Study of Molecular Motors - p. 516 24. Chapter Eighteen: Enzyme Kinetics in Femtoliter Arrays - p. 539 25. Author Index - p. 561 26. Subject Index - p. 587
Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases - p. xviii 8. Chapter Two: Single-Molecule Imaging With One Color Fluorescence - p. 32 9. Chapter Three: Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy - p. 51 10. Chapter Four: Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging - p. 82 11. Chapter Five: Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy - p. 104 12. Chapter Six: How to Measure Separations and Angles Between Intramolecular Fluorescent Markers - p. 145 13. Chapter Seven: Precisely and Accurately Inferring Single-Molecule Rate Constants - p. 185 14. Chapter Eight: Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout - p. 225 15. Chapter Nine: Putting Humpty–Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome - p. 256 16. Chapter Ten: Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins - p. 283 17. Chapter Eleven: Single-Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases - p. 315 18. Chapter Twelve: Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET - p. 351 19. Chapter Thirteen: Methods for Investigating DNA Accessibility with Single Nucleosomes - p. 378 20. Chapter Fourteen: Single-Molecule Fluorescence Studies of Fast Protein Folding - p. 415 21. Chapter Fifteen: Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules - p. 459 22. Chapter Sixteen: A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously - p. 486 23. Chapter Seventeen: Interferometric Scattering Microscopy for the Study of Molecular Motors - p. 516 24. Chapter Eighteen: Enzyme Kinetics in Femtoliter Arrays - p. 539 25. Author Index - p. 561 26. Subject Index - p. 587
