Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications.
Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.
1. Cover - p. i 2. Title page - p. ii 3. Table of Contents - p. ii 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Advanced sample environments and sample requirements for biological SAXS - p. 1 8. Chapter Two: Contrast variation SAXS: Sample preparation protocols, experimental procedures, and data analysis - p. 41 9. Chapter Three: Deuteration for biological SANS: Case studies, success and challenges in chemistry and biology - p. 85 10. Chapter Four: Planning, executing and assessing the validity of SANS contrast variation experiments - p. 127 11. Chapter Five: Technical considerations for small-angle neutron scattering from biological macromolecules in solution: Cross sections, contrasts, instrument setup and measurement - p. 157 12. Chapter Six: Size exclusion chromatography coupled small angle X-ray scattering with tandem multiangle light scattering at the SIBYLS beamline - p. 191 13. Chapter Seven: SEC-SAXS: Experimental set-up and software developments build up a powerful tool - p. 221 14. Chapter Eight: Stopped-flow-time-resolved SAXS for studies of ligand-driven protein dimerization - p. 251 15. Chapter Nine: Time-resolved small-angle neutron scattering (TR-SANS) for structural biology of dynamic systems: Principles, recent developments, and practical guidelines - p. 263 16. Chapter Ten: Protein fibrillation from another small angle: Sample preparation and SAXS data collection - p. 291 17. Chapter Eleven: High-pressure SAXS, deep life, and extreme biophysics - p. 323 18. Chapter Twelve: Characterization of biological materials with soft X-ray scattering - p. 357 19. Chapter Thirteen: Scattering measurements on lipid membrane structures - p. 385 20. Chapter Fourteen: Studying integral membrane protein by SANS using stealth reconstitution systems - p. 417 21. Chapter Fifteen: Predicting solution scattering patterns with explicit-solvent molecular simulations - p. 433 22. Chapter Sixteen: From dilute to concentrated solutions of intrinsically disordered proteins: Sample preparation and data collection - p. 457 23. Chapter Seventeen: Deriving RNA topological structure from SAXS - p. 479 24. Chapter Eighteen: Disentangling polydisperse biomolecular systems by Chemometrics decomposition of SAS data - p. 531
Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications.
Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.
1. Cover - p. i 2. Title page - p. ii 3. Table of Contents - p. ii 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Advanced sample environments and sample requirements for biological SAXS - p. 1 8. Chapter Two: Contrast variation SAXS: Sample preparation protocols, experimental procedures, and data analysis - p. 41 9. Chapter Three: Deuteration for biological SANS: Case studies, success and challenges in chemistry and biology - p. 85 10. Chapter Four: Planning, executing and assessing the validity of SANS contrast variation experiments - p. 127 11. Chapter Five: Technical considerations for small-angle neutron scattering from biological macromolecules in solution: Cross sections, contrasts, instrument setup and measurement - p. 157 12. Chapter Six: Size exclusion chromatography coupled small angle X-ray scattering with tandem multiangle light scattering at the SIBYLS beamline - p. 191 13. Chapter Seven: SEC-SAXS: Experimental set-up and software developments build up a powerful tool - p. 221 14. Chapter Eight: Stopped-flow-time-resolved SAXS for studies of ligand-driven protein dimerization - p. 251 15. Chapter Nine: Time-resolved small-angle neutron scattering (TR-SANS) for structural biology of dynamic systems: Principles, recent developments, and practical guidelines - p. 263 16. Chapter Ten: Protein fibrillation from another small angle: Sample preparation and SAXS data collection - p. 291 17. Chapter Eleven: High-pressure SAXS, deep life, and extreme biophysics - p. 323 18. Chapter Twelve: Characterization of biological materials with soft X-ray scattering - p. 357 19. Chapter Thirteen: Scattering measurements on lipid membrane structures - p. 385 20. Chapter Fourteen: Studying integral membrane protein by SANS using stealth reconstitution systems - p. 417 21. Chapter Fifteen: Predicting solution scattering patterns with explicit-solvent molecular simulations - p. 433 22. Chapter Sixteen: From dilute to concentrated solutions of intrinsically disordered proteins: Sample preparation and data collection - p. 457 23. Chapter Seventeen: Deriving RNA topological structure from SAXS - p. 479 24. Chapter Eighteen: Disentangling polydisperse biomolecular systems by Chemometrics decomposition of SAS data - p. 531
