Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study.
Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed.
1. Chapter 1. Introduction: Stem cells and their application in research and therapy - p. 6 2. Chapter 2. Animal stem cells: Extraction, expansion, and cryopreservation - p. 36 3. Chapter 3. Protocols in stem cell culture - p. 41 4. Chapter 4. Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques - p. 71 5. Isolation and characterization of c-kit+ stem cells from rat full-term gestation amniotic fluid - p. 95 6. Chapter 6. Human induced pluripotent stem cells (hiPSCs): Generation, characterization, and differentiation - p. 123 7. Confirmation of stem cells pluripotency via teratoma formation in an animal model - p. 143 8. Chapter 8. Gene editing for stem cells by CRISPR-Cas9 - p. 153 9. Chapter 9. Hypoxic environment: A new solution to stem cell culture - p. 180 10. Chapter 10. Mouse embryonic stem cell-derived cerebral organoids - p. 189 11. Chapter 11. Standardized practice of stem cell production - p. 201 12. Index - p. 219
Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study.
Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed.
1. Chapter 1. Introduction: Stem cells and their application in research and therapy - p. 6 2. Chapter 2. Animal stem cells: Extraction, expansion, and cryopreservation - p. 36 3. Chapter 3. Protocols in stem cell culture - p. 41 4. Chapter 4. Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques - p. 71 5. Isolation and characterization of c-kit+ stem cells from rat full-term gestation amniotic fluid - p. 95 6. Chapter 6. Human induced pluripotent stem cells (hiPSCs): Generation, characterization, and differentiation - p. 123 7. Confirmation of stem cells pluripotency via teratoma formation in an animal model - p. 143 8. Chapter 8. Gene editing for stem cells by CRISPR-Cas9 - p. 153 9. Chapter 9. Hypoxic environment: A new solution to stem cell culture - p. 180 10. Chapter 10. Mouse embryonic stem cell-derived cerebral organoids - p. 189 11. Chapter 11. Standardized practice of stem cell production - p. 201 12. Index - p. 219
