Steroid Biochemistry, Volume 688 in the Methods of Enzymology series, highlights new advances in the field, containing chapters on a variety of timely topics, including Cytochrome P450 Enzyme, Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5), Steroid 17 alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1), Enzymes of Estrogen Biosynthesis, Aromatase and Steroid Sulfatase, Estrogenic 17b-Hydroxysteroid Dehydrogenase, Hydroxysteroid Dehydrogenases (HSD), 3a-Hydroxyssteroid Dehydrogenase, Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1, 3b-Hydroxysteroid Dehydrogenase Type 2, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 - p. iii 9. Section 1 Cytochrome P450 enzymes - p. iii 10. Chapter One: Generation of human steroidogenic cytochrome P450 enzymes for structural and functional characterization - p. 3 11. Chapter Two: Steroid 17α-hydroxylase/17, 20-lyase (cytochrome P450 17A1) - p. 39 12. Section 2 - p. 63 13. Section 2 Enzymes of estrogen biosynthesis - p. 63 14. Chapter Three: Aromatase and steroid sulfatase from human placenta - p. 67 15. Section 3 - p. 84 16. Section 3 Hydroxysteroid dehydrogenases (HSD) - p. 84 17. Chapter Four: Approaches to assessing 3β-hydroxysteroid dehydrogenase-1 - p. 89 18. Chapter Five: In vitro methods to assess 11β-hydroxysteroid dehydrogenase type 1 activity - p. 121 19. Chapter Six: In vitro methods to assess 11β-hydroxysteroid dehydrogenase type 2 activity - p. 167 20. Chapter Seven: 17β-Hydroxysteroid dehydrogenases types 1 and 2: Enzymatic assays based on radiometric and mass-spectrometric detection - p. 203 21. Section 4 - p. 234 22. Section 4 Steroid reductases - p. 234 23. Chapter Eight: Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5α-reductases - p. 237 24. Chapter Nine: Bacterial expression, purification, and reconstitution of human steroid 5α-reductases in phospholipid liposomes and nanodiscs - p. 263 25. Chapter Ten: Steroid 5β-reductase (AKR1D1): Purification and characterization - p. 277 26. Chapter Eleven: Aldo keto-reductase family 1C members 1 through 4 recombinant enzyme purification and enzyme assay - p. 303 27. Section 5 - p. 329 28. Section 5 Steroid conjugating enzymes - p. 329 29. Chapter Twelve: Human cytosolic steroid sulfotransferases: Versatile and rapid activity assays - p. 333 30. Section 6 - p. 353 31. Section 6 Steroid analytical chemistry - p. 353 32. Chapter Thirteen: High sensitivity LC-MS methods for quantitation of hydroxy- and keto-androgens - p. 357 33. Chapter Fourteen: Detection and quantification of UV-transparent keto-androgens by dinitrophenylhydrazine derivatization for discontinuous kinetic assays - p. 379 34. Chapter Fifteen: Investigating the biosynthesis and metabolism of 11β-hydroxyandrostenedione - p. 389 35. Chapter Sixteen: Ultrasensitive quantification of estrogens in serum and plasma by liquid chromatography-tandem mass spectrometry - p. 435
Steroid Biochemistry, Volume 688 in the Methods of Enzymology series, highlights new advances in the field, containing chapters on a variety of timely topics, including Cytochrome P450 Enzyme, Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5), Steroid 17 alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1), Enzymes of Estrogen Biosynthesis, Aromatase and Steroid Sulfatase, Estrogenic 17b-Hydroxysteroid Dehydrogenase, Hydroxysteroid Dehydrogenases (HSD), 3a-Hydroxyssteroid Dehydrogenase, Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1, 3b-Hydroxysteroid Dehydrogenase Type 2, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 - p. iii 9. Section 1 Cytochrome P450 enzymes - p. iii 10. Chapter One: Generation of human steroidogenic cytochrome P450 enzymes for structural and functional characterization - p. 3 11. Chapter Two: Steroid 17α-hydroxylase/17, 20-lyase (cytochrome P450 17A1) - p. 39 12. Section 2 - p. 63 13. Section 2 Enzymes of estrogen biosynthesis - p. 63 14. Chapter Three: Aromatase and steroid sulfatase from human placenta - p. 67 15. Section 3 - p. 84 16. Section 3 Hydroxysteroid dehydrogenases (HSD) - p. 84 17. Chapter Four: Approaches to assessing 3β-hydroxysteroid dehydrogenase-1 - p. 89 18. Chapter Five: In vitro methods to assess 11β-hydroxysteroid dehydrogenase type 1 activity - p. 121 19. Chapter Six: In vitro methods to assess 11β-hydroxysteroid dehydrogenase type 2 activity - p. 167 20. Chapter Seven: 17β-Hydroxysteroid dehydrogenases types 1 and 2: Enzymatic assays based on radiometric and mass-spectrometric detection - p. 203 21. Section 4 - p. 234 22. Section 4 Steroid reductases - p. 234 23. Chapter Eight: Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5α-reductases - p. 237 24. Chapter Nine: Bacterial expression, purification, and reconstitution of human steroid 5α-reductases in phospholipid liposomes and nanodiscs - p. 263 25. Chapter Ten: Steroid 5β-reductase (AKR1D1): Purification and characterization - p. 277 26. Chapter Eleven: Aldo keto-reductase family 1C members 1 through 4 recombinant enzyme purification and enzyme assay - p. 303 27. Section 5 - p. 329 28. Section 5 Steroid conjugating enzymes - p. 329 29. Chapter Twelve: Human cytosolic steroid sulfotransferases: Versatile and rapid activity assays - p. 333 30. Section 6 - p. 353 31. Section 6 Steroid analytical chemistry - p. 353 32. Chapter Thirteen: High sensitivity LC-MS methods for quantitation of hydroxy- and keto-androgens - p. 357 33. Chapter Fourteen: Detection and quantification of UV-transparent keto-androgens by dinitrophenylhydrazine derivatization for discontinuous kinetic assays - p. 379 34. Chapter Fifteen: Investigating the biosynthesis and metabolism of 11β-hydroxyandrostenedione - p. 389 35. Chapter Sixteen: Ultrasensitive quantification of estrogens in serum and plasma by liquid chromatography-tandem mass spectrometry - p. 435
