Replication-Coupled Repair, Volume 661 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including the Repair of replication-born DNA breaks by sister chromatid recombination, High resolution and high throughput DNA cyclization measurements to interrogate DNA bendability, A programmable detection method for genomic signatures: from disease diagnosis to genome editing, Characterization of the telomerase modulating activities of yeast DNA helicases, Eukaryotic DNA replication with purified budding yeast proteins, Single molecule studies of yeast Rad51 paralogs, Light activation and deactivation of Cas9 for DNA repair studies, and more.
Other chapters explore MIDAS: Direct sequencing to map mitotic DNA synthesis and common fragile sites at high precision, Studying the DNA damage response in embryonic systems, GLASS-ChIP to map Mre11 cleavage sites in the human genome, New chemical biology approaches to trap reaction intermediates in living cells, Single-molecule imaging approaches for monitoring replication fork conflicts at genomic DNA G4 structures and R-loops in human cells, Monitoring the replication of structured DNA through heritable epigenetic change, Visualizing replication fork encounters with DNA interstrand crosslinks, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Eukaryotic DNA replication with purified budding yeast proteins - p. iii 9. Monitoring the replication of structured DNA through heritable epigenetic change - p. iii 10. Visualizing replication fork encounters with DNA interstrand crosslinks - p. iii 11. Single-molecule imaging of replication fork conflicts at genomic DNA G4 structures in human cells - p. iii 12. Studying the DNA damage response in embryonic systems - p. iii 13. Analysis of repair of replication-born double-strand breaks by sister chromatid recombination in yeast - p. iii 14. Determining the kinetics of break-induced replication (BIR) by the assay for monitoring BIR elongation rate (AMBER) - p. iii 15. Genomic mapping of DNA-repair reaction intermediates in living cells with engineered DNA structure-trap proteins - p. iii 16. A mapping platform for mitotic crossover by single-cell multi-omics - p. iii 17. Characterization of DNA-PK-bound end fragments using GLASS-ChIP - p. iii 18. Light activation and deactivation of Cas9 for DNA repair studies - p. iii 19. A detection method for the capture of genomic signatures: From disease diagnosis to genome editing - p. iii 20. A method to sequence genomic sites of mitotic DNA synthesis in mammalian cells - p. iii 21. Loop-seq: A high-throughput technique to measure the mesoscale mechanical properties of DNA - p. iii 22. Characterization of the telomerase modulating activities of yeast DNA helicases - p. iii 23. Single-molecule studies of yeast Rad51 paralogs - p. iii 24. Synthesis and polymerase bypass studies of DNA-peptide and DNA-protein conjugates - p. iii 25. An efficient chemical screening method for structure-based inhibitors to nucleic acid enzymes targeting the DNA repair-replication interface and SARS CoV-2 - p. iii
Replication-Coupled Repair, Volume 661 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including the Repair of replication-born DNA breaks by sister chromatid recombination, High resolution and high throughput DNA cyclization measurements to interrogate DNA bendability, A programmable detection method for genomic signatures: from disease diagnosis to genome editing, Characterization of the telomerase modulating activities of yeast DNA helicases, Eukaryotic DNA replication with purified budding yeast proteins, Single molecule studies of yeast Rad51 paralogs, Light activation and deactivation of Cas9 for DNA repair studies, and more.
Other chapters explore MIDAS: Direct sequencing to map mitotic DNA synthesis and common fragile sites at high precision, Studying the DNA damage response in embryonic systems, GLASS-ChIP to map Mre11 cleavage sites in the human genome, New chemical biology approaches to trap reaction intermediates in living cells, Single-molecule imaging approaches for monitoring replication fork conflicts at genomic DNA G4 structures and R-loops in human cells, Monitoring the replication of structured DNA through heritable epigenetic change, Visualizing replication fork encounters with DNA interstrand crosslinks, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Eukaryotic DNA replication with purified budding yeast proteins - p. iii 9. Monitoring the replication of structured DNA through heritable epigenetic change - p. iii 10. Visualizing replication fork encounters with DNA interstrand crosslinks - p. iii 11. Single-molecule imaging of replication fork conflicts at genomic DNA G4 structures in human cells - p. iii 12. Studying the DNA damage response in embryonic systems - p. iii 13. Analysis of repair of replication-born double-strand breaks by sister chromatid recombination in yeast - p. iii 14. Determining the kinetics of break-induced replication (BIR) by the assay for monitoring BIR elongation rate (AMBER) - p. iii 15. Genomic mapping of DNA-repair reaction intermediates in living cells with engineered DNA structure-trap proteins - p. iii 16. A mapping platform for mitotic crossover by single-cell multi-omics - p. iii 17. Characterization of DNA-PK-bound end fragments using GLASS-ChIP - p. iii 18. Light activation and deactivation of Cas9 for DNA repair studies - p. iii 19. A detection method for the capture of genomic signatures: From disease diagnosis to genome editing - p. iii 20. A method to sequence genomic sites of mitotic DNA synthesis in mammalian cells - p. iii 21. Loop-seq: A high-throughput technique to measure the mesoscale mechanical properties of DNA - p. iii 22. Characterization of the telomerase modulating activities of yeast DNA helicases - p. iii 23. Single-molecule studies of yeast Rad51 paralogs - p. iii 24. Synthesis and polymerase bypass studies of DNA-peptide and DNA-protein conjugates - p. iii 25. An efficient chemical screening method for structure-based inhibitors to nucleic acid enzymes targeting the DNA repair-replication interface and SARS CoV-2 - p. iii
