The intestine is among the leading organs, in which several cutting edge in vitro and in vivo research tools and approaches have recently been developed and used to investigate stem cell biology/function, and the potential applications of stem cells in the treatment of intestinal diseases. These cutting-edge research tools and approaches involve human and murine organoid cultures, genetic editing in vitro and in vivo, human induced pluripotent cell (iPS cell) models of disease, haploid cells for genetic as well as compound screening paradigms, genetically engineered mice, and stem cell transplantation to cure diseases.
Stem Cell Innovation in Health and Disease: Volume 1: The Intestine contains two major sections describing cutting edge research for understanding stem cell functions in the intestine, and for developing methods to bring stem cells from bench to bedside; respectively. Each section includes insights ranging from using mouse and human organoid cultures, genetic editing in vitro and in vivo, and human induced pluripotent cells (iPSCs) to study stem cell functions and model intestinal diseases, through the cutting-edge research, including the potential application of iPSCs, ESCs and blood stem cells (stem cell transplants) in the treatment of intestinal diseases/disorders. This volume, therefore, discusses the fact-based promise of stem cells and regenerative medicine in the intestine in the real world.
1. The Intestine - p. i 2. Cover - p. i 3. Title page - p. ii 4. Copyright - p. iv 5. Dedication - p. v 6. Epigraph - p. vii 7. Contents - p. viii 8. List of contributors - p. xv 9. About the editors - p. xvii 10. Foreword - p. xix 11. Preface - p. xxii 12. Acknowledgments - p. xxvii 13. Chapter 1 Cutting-edge tools and approaches for stem cell research and application in intestinal diseases - p. 1 14. Chapter 2 Organotypic intestinal cell culture as a new modality for intestinal function and cellular processes - p. 5 15. Chapter 3 Role of human gastrointestinal organoids in discovery and translational medicine - p. 29 16. Chapter 4 Engineered stem cells combine stem cell and gene therapy approaches to move intestine therapy from bench to bed - p. 59 17. Chapter 5 Role of CRISPR/Cas9 and other gene editing/engineering technology in intestine diseases - p. 75 18. Chapter 6 Application of new approaches for intestinal repair and regeneration via stem cell–based tissue engineering - p. 87 19. Chapter 7 Induced pluripotent stem cells in intestinal diseases - p. 101 20. Chapter 8 Potential of embryonic stem cells for treating intestinal diseases - p. 123 21. Chapter 9 Stem-cell therapy with bone marrow (hematopoietic) stem cells for intestinal diseases - p. 131 22. Chapter 10 Role of mesenchymal and other stem cell therapy in intestinal diseases - p. 147 23. Chapter 11 General discussion, conclusion remarks, and future directions - p. 165 24. Index - p. 173
The intestine is among the leading organs, in which several cutting edge in vitro and in vivo research tools and approaches have recently been developed and used to investigate stem cell biology/function, and the potential applications of stem cells in the treatment of intestinal diseases. These cutting-edge research tools and approaches involve human and murine organoid cultures, genetic editing in vitro and in vivo, human induced pluripotent cell (iPS cell) models of disease, haploid cells for genetic as well as compound screening paradigms, genetically engineered mice, and stem cell transplantation to cure diseases.
Stem Cell Innovation in Health and Disease: Volume 1: The Intestine contains two major sections describing cutting edge research for understanding stem cell functions in the intestine, and for developing methods to bring stem cells from bench to bedside; respectively. Each section includes insights ranging from using mouse and human organoid cultures, genetic editing in vitro and in vivo, and human induced pluripotent cells (iPSCs) to study stem cell functions and model intestinal diseases, through the cutting-edge research, including the potential application of iPSCs, ESCs and blood stem cells (stem cell transplants) in the treatment of intestinal diseases/disorders. This volume, therefore, discusses the fact-based promise of stem cells and regenerative medicine in the intestine in the real world.
1. The Intestine - p. i 2. Cover - p. i 3. Title page - p. ii 4. Copyright - p. iv 5. Dedication - p. v 6. Epigraph - p. vii 7. Contents - p. viii 8. List of contributors - p. xv 9. About the editors - p. xvii 10. Foreword - p. xix 11. Preface - p. xxii 12. Acknowledgments - p. xxvii 13. Chapter 1 Cutting-edge tools and approaches for stem cell research and application in intestinal diseases - p. 1 14. Chapter 2 Organotypic intestinal cell culture as a new modality for intestinal function and cellular processes - p. 5 15. Chapter 3 Role of human gastrointestinal organoids in discovery and translational medicine - p. 29 16. Chapter 4 Engineered stem cells combine stem cell and gene therapy approaches to move intestine therapy from bench to bed - p. 59 17. Chapter 5 Role of CRISPR/Cas9 and other gene editing/engineering technology in intestine diseases - p. 75 18. Chapter 6 Application of new approaches for intestinal repair and regeneration via stem cell–based tissue engineering - p. 87 19. Chapter 7 Induced pluripotent stem cells in intestinal diseases - p. 101 20. Chapter 8 Potential of embryonic stem cells for treating intestinal diseases - p. 123 21. Chapter 9 Stem-cell therapy with bone marrow (hematopoietic) stem cells for intestinal diseases - p. 131 22. Chapter 10 Role of mesenchymal and other stem cell therapy in intestinal diseases - p. 147 23. Chapter 11 General discussion, conclusion remarks, and future directions - p. 165 24. Index - p. 173
